Sökning: "metabolic engineering"

Visar resultat 6 - 10 av 169 avhandlingar innehållade orden metabolic engineering.

  1. 6. Evaluation of precursor and cofactor engineering strategies influencing fatty acid metabolism in Saccharomyces cerevisiae

    Författare :Alexandra Linda Bergman; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; metabolic engineering; fatty acids; oleochemicals; NADPH; Sustainability; phosphoketolase; acetyl-CoA; transcription factor;

    Sammanfattning : If humanity is to reduce the rate of climate change, it is essential that our societies switch to a more sustainable production of fuels and chemicals, which in turn depends on technological development. Oleochemical production via microbial catalysts – such as the yeast Saccharomyces cerevisiae – can use a considerably broader range of renewable substrates compared to the conventional production processes. LÄS MER

  3. 7. Probing Control of Glucose Feeding in Escherichia coli Cultivations

    Författare :Mats Åkesson; Institutionen för reglerteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Automatiska system; control engineering; robotics; Automation; dissolved oxygen; acetate; glucose feeding; robotteknik; Escherichia coli; Process control; fed-batch cultivation; reglerteknik; Biotechnology; Bioteknik;

    Sammanfattning : Production of many proteins can today be made using genetically modified organisms. One of the most frequently used host organisms is the bacterium Escherichia coli. A difficulty encountered in cultivations of E. coli is the accumulation of the metabolic by-product acetate which inhibits cell growth and production of a desired protein. LÄS MER

  4. 8. Metabolic analysis of shikimic acid producing Escherichia coli

    Författare :Louise Johansson; Avdelningen för kemiteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Biokemi; metabolism; Chemical technology and engineering; transcriptome analysis; phosphorus-limitation; carbon-limitation; fed-batch; chemostat; batch; fermentation technology; Escherichia coli; shikimic acid; Biotechnology; Bioteknik; Metabolism; Biochemistry; principal component analysis; 2D-fluorescence spectrophotometry; metabolic flux analysis; carbon-rich;

    Sammanfattning : Shikimic acid production using recombinant E.coli has been developed during the past 15 years due to the increased demand of this product, primarily as a starting material for the anti-influensa medicine Tamiflu?. A detailed metabolic analysis of a shikimic acid producing E. LÄS MER

  5. 9. Crossing the river by feeling the stones : Approaches to Sustainable Urban Development in China

    Författare :Hongling Liu; Björn Frostell; Dick Urban Vestbro; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY;

    Sammanfattning : China already has the highest numbe of,and largest cities in its history. According to recent statistics, Chinese cities were home to 52.6% of China’s total population at the end of 2012. LÄS MER

  7. 10. Engineering enzymes towards biotherapeutic applications using ancestral sequence reconstruction

    Författare :Natalie Hendrikse; Per-Olof Syrén; Johan Rockberg; Erik Nordling; Reinhard Sterner; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; enzyme engineering; enzyme evolution; ancestral sequence reconstruction; therapeutic enzymes; terpene cyclases; ammonia-lyases; sulfatases; lysosomal enzymes; Kemi; Chemistry;

    Sammanfattning : Enzymes are versatile biocatalysts that fulfill essential functions in all forms of life and, therefore, play an important role in health and disease. One specific application of enzymes in life science is their use as biopharmaceuticals, which typically benefits from high catalytic activity and stability. LÄS MER