Sökning: "drug discovery"

Visar resultat 16 - 20 av 289 avhandlingar innehållade orden drug discovery.

  1. 16. Bringing Structure to Drug Discovery

    Författare :Hermina Wieske; Máté Erdélyi; Craig Butts; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Drug discovery is aided by structural information. The type of structural information needed depends on the question at hand. The methods that can be used to determine the absolute configuration of a newly synthesised compound are different from those needed to study ligand binding. LÄS MER

  3. 17. Biosensor Studies of Ligand Interactions with Structurally Flexible Enzymes : Applications for Antiviral Drug Development

    Författare :Matthis Geitmann; U. Helena Danielson; Marc H. V. van Regenmortel; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; SPR biosensor; HIV-1 reverse transcriptase; HCMV protease; interaction kinetics; drug discovery; non-nucleoside inhibitor; resistance; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The use of a surface plasmon biosensor fills a missing link in kinetic studies of enzymes, since it measures directly the interaction between biomolecules and allows determination of parameters that are determined only indirectly in activity assays. The present thesis deals with kinetic and dynamic aspects of ligand binding to two viral enzymes: the human cytomegalovirus (HCMV) protease and the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). LÄS MER

  4. 18. Electrospray Ionization Mass Spectrometry for Determination of Noncovalent Interactions in Drug Discovery

    Författare :Kurt Benkestock; Johan Roeraade; Renato Zenobi; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; electrospray ionization; drug discovery; noncovalent interaction; complexes; human serum albumin HSA ; fatty acid binding protein FABP ; ribonuclease; ligand screening; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Noncovalent interactions are involved in many biological processes in which biomolecules bind specifically and reversibly to a partner. Often, proteins do not have a biological activity without the presence of a partner, a ligand. LÄS MER

  5. 19. Proteomic and Functional Analysis of In Vitro Systems for Studies of Drug Disposition in the Human Small Intestine and Liver

    Författare :Magnus Ölander; Per Artursson; Pär Matsson; Edward L. LeCluyse; Volker Lauschke; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; drug disposition; ADMET; drug transport; drug metabolism; hepatotoxicity; small intestine; liver; caco-2; human hepatocytes; cryopreservation; apoptosis; liver zonation; non-parenchymal cells; Farmaceutisk vetenskap; Pharmaceutical Science;

    Sammanfattning : To reach the bloodstream, an orally administered drug must be absorbed through the small intestine and avoid extensive clearance in the liver. Estimating these parameters in vitro is therefore important in drug discovery and development. LÄS MER

  7. 20. Molecular Approaches to Explore Drug-Target Interactions

    Författare :Abdullah Al-Amin; Ulf Landegren; Christopher Schofield; Uppsala universitet; []
    Nyckelord :Drug discovery; target engagement; target engagement-mediated amplification; cellular thermal shift assay; proximity extension assay; in situ PLA; high-content imaging; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Improved means to assess the clinical potential of drug candidates can critically influence development of new therapeutic entities, a central aim in medical life science. Drug discovery and development relies on construction and selection of small organic compounds or biological agents that bind targets of interest. LÄS MER