Sökning: "cellular thermal shift assay"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden cellular thermal shift assay.

  1. 1. Proximity Extension Assay : a universal tool for proteomics in precision medicine

    Författare :Phathutshedzo M. Muthelo; Ulf Landegren; Jochen Schwenk; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proximity Extension Assay; Precision medicine; Assay development; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : Precision medicine aims to utilise biomarkers to ensure the right treatment is given to the right patient at the right time. In pursuit of this goal, sensitive protein detection is essential to improve opportunities for discovery, verification, validation and development of valuable protein biomarkers. LÄS MER

  3. 2. Molecular Approaches to Explore Drug-Target Interactions

    Författare :Abdullah Al-Amin; Ulf Landegren; Christopher Schofield; Uppsala universitet; []
    Nyckelord :Drug discovery; target engagement; target engagement-mediated amplification; cellular thermal shift assay; proximity extension assay; in situ PLA; high-content imaging; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Improved means to assess the clinical potential of drug candidates can critically influence development of new therapeutic entities, a central aim in medical life science. Drug discovery and development relies on construction and selection of small organic compounds or biological agents that bind targets of interest. LÄS MER

  4. 3. The cellular thermal shift assay (CETSA) for elucidating drug mechanism of action and resistance in cancer

    Författare :Smaranda Bacanu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : A complete understanding over drug mechanism of action (MoA) is important when attempting to predict treatment outcome or the presence of resistance in patients. Despite decades of scientific efforts, the MoAs of even some of the oldest and most utilized drugs in cancer therapy today remain only partially understood, while resistance continues to be a frequent and often unpredictable occurrence. LÄS MER

  5. 4. Determining Ligand- and Lipid- Interactions of SLC Transporters

    Författare :Yurie Chatzikyriakidou; David Drew; Robert Fredriksson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Solute carrier transporter; nucleotide-sugar transporter; SLC35; sodium proton exchanger; SLC9B2; thermal stability assay; membrane transport; Synaptic vesicle protein 2; Biochemistry; biokemi;

    Sammanfattning : Solute carrier transporters (SLCs) mediate the inter- and intra- cellular trafficking of a plethora of substrates and are essential to cell homeostasis. Despite their importance to human physiology and their potential as therapeutic targets, many SLCs are considered orphans as the physiological substrate has not been experimentally determined. LÄS MER

  7. 5. Targeting polo-like kinase 1 in pediatric leukemia

    Författare :Oksana Goroshchuk; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Acute lymphoblastic leukemia (ALL) is one of the most common cancers among children in the world, with almost 90% complete remission rate after the primary treatment. However, some patients are resistant to treatment and some relapse later. Moreover, existing treatments are toxic and induce life-long adverse effects. LÄS MER