Sökning: "HCMV protease"

Hittade 2 avhandlingar innehållade orden HCMV protease.

1. 1. Biosensor Studies of Ligand Interactions with Structurally Flexible Enzymes : Applications for Antiviral Drug Development

   Författare :Matthis Geitmann; U. Helena Danielson; Marc H. V. van Regenmortel; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; SPR biosensor; HIV-1 reverse transcriptase; HCMV protease; interaction kinetics; drug discovery; non-nucleoside inhibitor; resistance; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : The use of a surface plasmon biosensor fills a missing link in kinetic studies of enzymes, since it measures directly the interaction between biomolecules and allows determination of parameters that are determined only indirectly in activity assays. The present thesis deals with kinetic and dynamic aspects of ligand binding to two viral enzymes: the human cytomegalovirus (HCMV) protease and the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). LÄS MER

3. 2. Novel Procedures for Identification and Characterization of Viral Proteases Inhibitors

   Författare :Angelica Ehrenberg; Helena Danielson; Celia Schiffer; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; drug discovery; viral proteases; inhibitors; Hepatitis C; NS3; cytomegalovirus; Biokemi; Biochemistry;

   Sammanfattning : Viral proteases are often considered to be attractive drug targets because of their crucial function in the viral replication machinery. In order to increase our knowledge of these important targets and to contribute to the discovery and development of new antiviral drugs, the proteases from hepatitis C virus (HCV) and human cytomegalovirus (HCMV) have been produced and their interactions with inhibitors and fragments have been characterized, using enzyme inhibition and SPR biosensor based interaction assay. LÄS MER