Sökning: "definitive endoderm"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden definitive endoderm.

  1. 1. Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm

    Författare :Karin Norrman; Stamcellscentrum (SCC); []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; human embryonic stem cells; diabetes; pancreas; definitive endoderm; foregut endoderm; pancreatic endoderm; constitutive promoter; ACTB; EF1alpha; CMV; PGK; UbC;

    Sammanfattning : Abstract More than 300 million people are suffering from diabetes worldwide and the numbers of diabetic patients are increasing. Today´s treatments mainly rely on daily insulin injections. This help people to control blood glucose levels but still many patients suffer from the many complications associated with diabetes. LÄS MER

  3. 2. FGF signaling in specification of hESC-derived definitive endoderm

    Författare :Jacqueline Ameri; Stamcellscentrum (SCC); []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : Diabetes affects around 200 million people worldwide. Curing diabetes would require the prevention of autoimmune destruction of beta cells and restoration of the beta cell mass restored either through regeneration or transplantation of the insulin producing cells. LÄS MER

  4. 3. Device Bonding and Antibody Binding: Developments for On-Chip Differentiation of Induced Pluripotent Stem Cells into Hepatocytes

    Författare :Philip Dalsbecker; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; liver-on-a-chip; organ-on-chip; PDMS; oxygen plasma; immunofluorescence; fluorescence microscopy; hepatocytes; iPSC; induced pluripotent stem cells; differentiation; on-chip differentiation; definitive endoderm;

    Sammanfattning : Abstract This thesis describes the bonding optimization of a liver-on-a-chip microfluidic device for evaluation of cultured induced pluripotent stem cell (iPSC)-derived definitive endoderm (DE) cells during their targeted differentiation into mature hepatocytes. Furthermore, it describes the optimization of a number of antibody assays for use during said differentiation, for the purpose of studying the cellular phenotype. LÄS MER

  5. 4. Human Embryonic Stem Cells: Directed Differentiation into Posterior Foregut Endoderm and a Functional Assay for Definitive Endoderm

    Författare :Martina Johannesson; Immunologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : A decade has passed since the first human embryonic stem cell (hESC) line was established, providing immense hope in curing diseases by cell replacement therapy. For instance, in the pancreas, the insulin-producing β-cells are damaged in diabetes patients, and a successful replacement of those cells could provide a cure. LÄS MER

  7. 5. Transcriptional profiling of human embryonic stem cells and their functional derivatives

    Författare :Jane Synnergren; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; transcription; differentiation; human embryonic stem cells; gene expression; Naturvetenskap;

    Sammanfattning : Human embryonic stem cells (hESCs) represent populations of pluripotent, undifferentiated cells with unlimited replication capacity, and with the ability to differentiate into any functional cell type in the human body. Based on these properties, hESCs and their derivatives provide unique model systems for basic research on embryonic development. LÄS MER