Sökning: "iPSC"

Visar resultat 1 - 5 av 26 avhandlingar innehållade ordet iPSC.

  1. 1. Human iPSC-based models of theCNS: attaining cellular biofidelitythrough conventional and advancedculture systems

    Författare :Dimitrios Voulgaris; Anna Herland; Susanna Narkhilakhi; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; neural stem cells; astrocytes; iPSC; hiAstrocytes; microphysiological systems; BBB; Bioteknologi; Biotechnology;

    Sammanfattning : Brain development is a highly orchestrated process that entails changes in microenvironmental cues and growth factor gradients, which set the tempo for proper development of the rudimentary structures of the brain and the generation of neurons,astrocytes, and oligodendrocytes. Another intricate feature of the brain is the bloodbrain barrier (BBB). LÄS MER

  3. 2. Genetics of Two Mendelian Traits and Validation of Induced Pluripotent Stem Cell (iPSC) Technology for Disease Modeling

    Författare :Doroteya Raykova; Niklas Dahl; Ann Nordgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Disease modeling; Mendelian disorders; iPSC; Whole exome sequencing; Transcriptome sequencing; Medical Science; Medicinsk vetenskap;

    Sammanfattning : Novel technologies for genome analysis have provided almost unlimited opportunities to uncover structural gene variants behind human disorders. Whole exome sequencing (WES) is especially useful for understanding rare Mendelian conditions, because it reduces the requirements for a priori clinical data, and can be applied on a small number of patients. LÄS MER

  4. 3. Device Bonding and Antibody Binding: Developments for On-Chip Differentiation of Induced Pluripotent Stem Cells into Hepatocytes

    Författare :Philip Dalsbecker; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; liver-on-a-chip; organ-on-chip; PDMS; oxygen plasma; immunofluorescence; fluorescence microscopy; hepatocytes; iPSC; induced pluripotent stem cells; differentiation; on-chip differentiation; definitive endoderm;

    Sammanfattning : Abstract This thesis describes the bonding optimization of a liver-on-a-chip microfluidic device for evaluation of cultured induced pluripotent stem cell (iPSC)-derived definitive endoderm (DE) cells during their targeted differentiation into mature hepatocytes. Furthermore, it describes the optimization of a number of antibody assays for use during said differentiation, for the purpose of studying the cellular phenotype. LÄS MER

  5. 4. Computational modelling in systems biology: from rewriting cell fates to detecting tumours

    Författare :Emil Andersson; Centrum för miljö- och klimatvetenskap (CEC); []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Beräkningsmodeller; Systembiologi; Genreguleringsnätverk; Cellomprogrammering; Energilandskap; iPSC; Agentbaserad modellering; T-cellsutveckling; Maskininlärning; Tumördiagnostik; Computational modelling; Systems biology; Gene regulatory network; Cell reprogramming; Energy landscape; iPSC; Agent-based modelling; Multi-scale modelling; T-cell development; Machine learning; Tumour diagnostics;

    Sammanfattning : Computational modelling is becoming an increasingly important tool in biological research. By performing computer simulations of models, it becomes possible to test theories about a biological system against experimental data. Simulations can also be used as a replacement for experiments otherwise unattainable. LÄS MER

  7. 5. Development and Applications of Microfluidic Devices for Liver-on-a-Chip Studies

    Författare :Philip Dalsbecker; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; Microphysiological system; Liver-on-a-Chip; Organ-on-a-chip; Hepatocytes; Induced pluripotent stem cells; iPSC; HepG2; LX-2; on-chip differentiation; PDMS; Microfabrication; Shear stress;

    Sammanfattning : Modeling the human physiology in vitro is a challenging task, yet one of importance for the development of drugs and the study of diseases. To better address the complexities of human organs, microfluidic systems called organs-on-chips are being developed to emulate organ-specific environments for cell culture. LÄS MER