Sökning: "Philip Dalsbecker"

Hittade 2 avhandlingar innehållade orden Philip Dalsbecker.

  1. 1. Development and Applications of Microfluidic Devices for Liver-on-a-Chip Studies

    Författare :Philip Dalsbecker; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; Microphysiological system; Liver-on-a-Chip; Organ-on-a-chip; Hepatocytes; Induced pluripotent stem cells; iPSC; HepG2; LX-2; on-chip differentiation; PDMS; Microfabrication; Shear stress;

    Sammanfattning : Modeling the human physiology in vitro is a challenging task, yet one of importance for the development of drugs and the study of diseases. To better address the complexities of human organs, microfluidic systems called organs-on-chips are being developed to emulate organ-specific environments for cell culture. LÄS MER

  3. 2. Device Bonding and Antibody Binding: Developments for On-Chip Differentiation of Induced Pluripotent Stem Cells into Hepatocytes

    Författare :Philip Dalsbecker; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; liver-on-a-chip; organ-on-chip; PDMS; oxygen plasma; immunofluorescence; fluorescence microscopy; hepatocytes; iPSC; induced pluripotent stem cells; differentiation; on-chip differentiation; definitive endoderm;

    Sammanfattning : Abstract This thesis describes the bonding optimization of a liver-on-a-chip microfluidic device for evaluation of cultured induced pluripotent stem cell (iPSC)-derived definitive endoderm (DE) cells during their targeted differentiation into mature hepatocytes. Furthermore, it describes the optimization of a number of antibody assays for use during said differentiation, for the purpose of studying the cellular phenotype. LÄS MER