Sökning: "assay development"

Visar resultat 11 - 15 av 595 avhandlingar innehållade orden assay development.

1. 11. Bioprocess development for biochemical conversion of lignocellulose

   Författare :Ruifei Wang; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Lignocellulose; strain engineering; stress tolerance; kinetic modelling; high gravity; scale-up; biofuels; SSCF; process development; Saccharomyces cerevisiae;

   Sammanfattning : Due to its low environmental impact and high maturity of the fuel ethanol market, lignocellulosic ethanol is a promising option for reducing the carbon footprint in the transport sector. The characteristics of lignocellulosic feedstocks, such as varied sugar composition, low sugar density, low solubility, recalcitrance to enzymatic degradation, and inhibitors formed during thermochemical pretreatment, have so far limited the production process, and costs for conversion of lignocellulosic materials to ethanol are still high. LÄS MER

3. 12. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors

   Författare :Knut Kotarsky; Slemhinnans immunologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemi; Metabolism; Biochemistry; metabolism; leukotrieneB4; thiazolidinedione; 9-HODE; fatty acid; luciferase; GFP; reporter gene; preclinical assay; receptor; GPCR;

   Sammanfattning : Seven-transmembrane, G-protein coupled receptors play a central role in physiology since they facilitate cell communication in multicellular organisms by recognition of a broad range of ligands. They also represent important drug targets. LÄS MER

4. 13. Testosterone and 17beta-oestradiol secretions in children and adolescents. Assay development, levels for comparison and clinical applications

   Författare :Carina Ankarberg-Lindgren; Göteborgs universitet; []
   Nyckelord :17beta-oestradiol; testosterone; sensitive assay; data for comparison; prepuberty; puberty; children; pubertal induction.;

   Sammanfattning : Accurate measurements of 17beta-oestradiol and testosterone are important in clinical settings of pubertal disorders and hormone replacement therapy among children. Most assays for measurement of these steroids are optimised for fertility investigations in adults and are not sensitive enough for quantification in children. LÄS MER

5. 14. Development and Application of Proximity Assays for Proteome Analysis in Medicine

   Författare :Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

   Sammanfattning : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. LÄS MER

7. 15. Understanding and manipulating primary cell walls in plant cell suspension cultures

   Författare :Felicia Leijon; Vincent Bulone; Katja Salomon Johansen; KTH; []
   Nyckelord :Callose synthase; carbohydrate-binding module; cell wall engineering; cellulose microfibril; cellulose nanocrystal; glycosyltransferase; mass spectrometry; plasmodesmata; Populus; primary cell wall; radiometric assay; spectrophotometric assay; Biotechnology; Bioteknologi;

   Sammanfattning : The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. LÄS MER