Sökning: "spectrophotometric assay"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden spectrophotometric assay.

  1. 1. Understanding and manipulating primary cell walls in plant cell suspension cultures

    Författare :Felicia Leijon; Vincent Bulone; Katja Salomon Johansen; KTH; []
    Nyckelord :Callose synthase; carbohydrate-binding module; cell wall engineering; cellulose microfibril; cellulose nanocrystal; glycosyltransferase; mass spectrometry; plasmodesmata; Populus; primary cell wall; radiometric assay; spectrophotometric assay; Biotechnology; Bioteknologi;

    Sammanfattning : The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. LÄS MER

  3. 2. Platelet Adhesion to Proteins in Microplates : Applications in Experimental and Clinical Research

    Författare :Andreas Eriksson; Per Whiss; Håkan Wallén; Linköpings universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; platelets; platelet adhesion; platelet assay; synergistic activation; thrombosis; anti-platelet treatment; Pharmacology; Farmakologi;

    Sammanfattning : Platelets are crucial for prevention of blood loss after vessel injury. Platelet adhesion to disrupted vessel walls is mediated by receptors such as the GPIb-IX-V complex that binds von Willebrand factor and the collagen-binding integrin α2β1. LÄS MER

  4. 3. Studies on the catalytic mechanism of butyrylcholinesterase and the development of a cholinesterase assay for clinical applications

    Författare :Håkan Eriksson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : This investigation is concerned with the kinetic behaviour of butyrylcholinesterase and the development of a simple, fast and reliable method for the determination of cholinesterases in whole blood.Steady state kinetic studies with selective inhibitors and with a number of substrates at several different conditions were used in an attempt to explain the observed substrate activation of horse serum butyrylcholinesterase. LÄS MER

  5. 4. Development of bioluminescent reagents for kinetic analysis and some applications in clinical chemistry

    Författare :Inger Styrélius; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Firefly luciferase and a bacterial luciferase system have been used to develop analytical methods suitable for use in clinical analysis.The influence of the level of NADH:FMN oxidoreductase on the kinetics of light emission in the bacterial luciferase system was studied. LÄS MER

  7. 5. Microbial Responses to Antibiotics – Stability of Resistance and Extended Potential of Targeting the Folate Synthesis

    Författare :Maria Jönsson; Göte Swedberg; Steven Meshnick; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; sulfonamide resistance; macrolide; commensal flora; substrate channeling; folate synthesis; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Sammanfattning : Resistance to antimicrobials is an increasing problem in the world of today, and develops faster than man can counter. It is therefore of importance to study metabolic pathways in order to develop new antibiotics, but also to understand how resistance spreads and stabilizes in microbial populations. LÄS MER