Sökning: "Proximity Extension Assay"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden Proximity Extension Assay.

  1. 1. Proximity Ligation Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Simon Fredriksson; Uppsala universitet.; [2002]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Sammanfattning : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. LÄS MER

  2. 2. Biomarkers for Better Understanding of the Pathophysiology and Treatment of Chronic Pain Investigations of Human Biofluids

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Anne-Li Lind; Uppsala universitet.; [2017]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; chronic pain; neuropathic pain; lumbar radicular pain; amyotrophic lateral sclerosis; radiculopathy; fibromyalgia; pathophysiology; spinal cord stimulation; mechanism of action; disc herniation; cerebrospinal fluid; plasma; biomarker; human; protein; chemokines; cytokines; inflammation; neuroinflammation; mass spectrometry; proximity ligation assay; proximity extension assay; antibody suspension bead array; protein profiling; molecular medicine; personalized medicine; Anestesiologi och intensivvård; Anaesthesiology and Intensive Care; Biomedical Laboratory Science; Biomedicinsk laboratorievetenskap; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry; Medicinsk vetenskap; Medical Science; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Chronic pain affects 20 % of the global population, causes suffering, is difficult to treat, and constitutes a large economic burden for society. So far, the characterization of molecular mechanisms of chronic pain-like behaviors in animal models has not translated into effective treatments. LÄS MER

  3. 3. Development and Application of Proximity Assays for Proteome Analysis in Medicine

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Felipe Marques Souza de Oliveira; Uppsala universitet.; [2018]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

    Sammanfattning : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. LÄS MER

  4. 4. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Tonge Ebai; Uppsala universitet.; [2017]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

    Sammanfattning : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. LÄS MER

  5. 5. Molecular Approaches to Explore Drug-Target Interactions

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Rasel Abdullah Al-Amin; Uppsala universitet.; [2019]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Drug discovery; target engagement; target engagement-mediated amplification; cellular thermal shift assay; proximity extension assay; in situ PLA; high-content imaging; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Improved means to assess the clinical potential of drug candidates can critically influence development of new therapeutic entities, a central aim in medical life science. Drug discovery and development relies on construction and selection of small organic compounds or biological agents that bind targets of interest. LÄS MER