Sökning: "Tillämpad biokemi"

Visar resultat 26 - 30 av 67 avhandlingar innehållade orden Tillämpad biokemi.

1. 26. Multimodal Interaction Chromatography for the Investigation and Separation of Proteins

   Författare :Kristian Kallberg; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Hydrophobic interaction chromatography; Multimodal chromatography; Glycosylation; Antibody; Hemoglobin; Protein purification.;

   Sammanfattning : Minor structural changes on the surface of proteins can have tremendous impact on their biological properties. When producing therapeutic proteins it is therefore important to verify that the generated protein is the desired one. LÄS MER

3. 27. Engineering of Human Fetal Hemoglobin

   Författare :Karin Kettisen; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fetal hemoglobin; protein engineering; recombinant protein production; protein purification; chromatography; oxygen carrier; redox reactions; net surface charge; DNA cleavage;

   Sammanfattning : In blood, the oxygen-transporting protein hemoglobin (Hb) governs the oxygenation of cells and tissues. Naturally, this protein has claimed a place in the center of the research field of artificial oxygen therapeutics. LÄS MER

4. 28. New Bioanalytical Approaches, Magneto Immunoassays and SIRE Technology Based Biosensors

   Författare :Kirstin Kriz; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemical technology; Biokemisk teknik; antigens; superparamagnetic nanoparticles; magnetic inductance transducer; competitive magneto immunoassay; enzymes; metabolites; differential measurements; SIRE; biosensor;

   Sammanfattning : This thesis presents two new technologies, SIRE technology based biosensors and magneto immunoassays, which can be used for bioanalytical applications. One of the main problems associated with biosensor technology is the instability of the recognition element since it is of biological origin and thus, has a limited lifetime. LÄS MER

5. 29. Biochemical and Physiological Characterization of Nonsymbiotic Plant Hemoglobins

   Författare :Nélida Leiva; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Hemoglobin; Non-symbiotic hemoglobin; Sugar beet; Ligand binding; Nitric oxide; Gene expression; Gene overexpression; Poplar; Arabidopsis; Lepidium campestre.;

   Sammanfattning : Hemoglobins (Hb) are usually associated with blood in humans. However, these proteins are widely distributed among living organisms. In plants the most known group are the leghemoglobins. Still, other Hbs that not participate in symbiosis are also found. LÄS MER

7. 30. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

   Författare :Gösta Lilius; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

   Sammanfattning : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. LÄS MER