Sökning: "Tillämpad biokemi"

Visar resultat 21 - 25 av 67 avhandlingar innehållade orden Tillämpad biokemi.

1. 21. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Författare :Florent Bernaudat; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER

3. 22. Phytoglobins: Elucidation of Structural, Functional and Practical Features of Non-Symbiotic Plant Hemoglobins

   Författare :Simon Christensen; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Hemoglobin; Fytoglobin; Hexakoordination; Karaktärisering; Protein design; Rekombinant protein production; Kromatografi; kristallografi; Dimerisering; Protein Dynamik;

   Sammanfattning : Phytoglobins (Pgb) (plant hemoglobins) are heme-containing proteins and less well known in comparison to the mammalian counterparts hemoglobin (Hb) andmyoglobin (Mb). They share the same secondary structure as other globin-related proteins, six to eight α-helices comprising the myoglobin-fold (Mb-fold) with one prosthetic heme moiety per subunit. LÄS MER

4. 23. HYDROPHOBIC PEPTIDE TAGS - As tools in bioseparation and investigation of recombinant proteins

   Författare :Sara Fexby; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; E. coli; Biochemical technology; Biokemisk teknik; fusion protein; tyrosine; hydrophobicity; Hydrophobic interaction chromatography; aqueous two-phase system;

   Sammanfattning : This thesis describes the possibility of using hydrophobic peptide tags as tools for bioseparation and for the investigation of recombinant proteins. The hydrophobicity of a protein can easily be changed by minor modifications, such as fusions of tyrosine-containing peptides. LÄS MER

5. 24. A radical view of haemoglobin - protein engineering and characterisation

   Författare :Marie Grey; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

   Sammanfattning : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. LÄS MER

7. 25. Effects of dietary fibre on the human metabolism and metabolome

   Författare :Anna Johansson Persson; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; arabinoxylan; b-glucan; dietary fibre; inflammation; LC-MS; metabolomics; oat; pectin; postprandial response; randomised controlled trial; rye; sugar beet fibre;

   Sammanfattning : It is well-known that dietary fibre can have a positive effect on the development of lifestyle-dependent diseases such as cardiovascular disease and type 2 diabetes. However, the effect may be different for different kinds of fibre and different sub-populations at risk. LÄS MER