Sökning: "Protein purification."

Visar resultat 1 - 5 av 265 avhandlingar innehållade orden Protein purification..

  1. 1. Recombinant amelogenin - Strategies for purification and protein processing

    Författare :Johan Svensson Bonde; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

    Sammanfattning : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. LÄS MER

  3. 2. Engineering of small IgG binding domains for antibody labelling and purification

    Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER

  4. 3. Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

    Författare :Julia Scheffel; Sophia Hober; Alois Jungbauer; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibodies; Antibody fragments; Biomanufacturing; Protein purification; Protein engineering; Protein A; ZCa; Calcium; Mild elution; Bioteknologi; Biotechnology;

    Sammanfattning : Antibodies are essential proteins in both our bodies and biotechnological research, and hold outstanding therapeutic value. The market for antibody-based therapeutics has grown exponentially during the last decades, owing to several advantages over small molecule drugs, such as fewer undesirable side effects associated with a higher target specificity. LÄS MER

  5. 4. Adipocyte phosphatases and the antilipolytic action of insulin

    Författare :Svante Resjö; Signaltransduktionsforskning; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein phosphorylation; protein purification; adipose tissue; PP2A; protein phosphatase; PDE3B; phosphodiesterase 3B; Insulin signalling; antilipolysis; Klinisk biologi; Clinical biology; PKB; protein kinase B;

    Sammanfattning : Adipose tissue is the main site of energy storage of the body and an important endocrine organ. Knowledge of the regulation of fat metabolism and the endocrine factors secreted by the adipocyte is crucial for the understanding of diseases such as obesity and diabetes. Insulin is the main antilipolytic hormone. LÄS MER

  7. 5. Human Aquaporins: Production, Characterization and Interactions

    Författare :Jennie Sjöhamn; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporins; membrane protein; protein production; protein characterization; protein:protein interactions; structural biochemistry;

    Sammanfattning : Membrane proteins are essential components of the cell and responsible for the communication with the outside environment and transport of molecules across the membrane. Water transport is facilitated by aquaporins, which are water selective transmembrane pores that serve to maintain cell homeostasis. LÄS MER