Sökning: "Single cell gel electrophoresis"

Visar resultat 1 - 5 av 14 avhandlingar innehållade orden Single cell gel electrophoresis.

1. 1. Evolving the Methodology for Detection of Primary DNA Damage : Development, adaptation and assessment of the single cell gel electrophoresis (comet) assay

   Författare :Erik Bivehed; Björn Hellman; Hanna Karlsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Comet assay; DNA damage; DNA strand breaks; Flash-comet; Genotoxicity; In vitro; Single cell gel electrophoresis; Polymerase assisted DNA damage assay PADDA ; TK6 cells; UCDAS; U937 cells; Toxikologi; Toxicology; Farmaceutisk vetenskap; Pharmaceutical Science;

   Sammanfattning : Deoxyribonucleic acid (DNA) is one of the most important molecules in nature. It is the fundamental carrier of evolutionary information and constitutes the genetic blueprint of all living organisms. Being the sole source of information, it is vital for the cell to transmit the correct genetic information from generation to generation. LÄS MER

3. 2. Application of Padlock Probe Based Nucleic Acid Analysis In Situ

   Författare :Sara Henriksson; Mats Nilsson; Ulf Landegren; Harvest Gu; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; rolling circle amplification; single cell gel electrophoresis assay; comet assay; Crimean Congo hemorrhagic fever virus; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

   Sammanfattning : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. LÄS MER

4. 3. Cell-protein-material Interactions on Bioceramics and Model Surfaces

   Författare :Åsa Rosengren; Sven Oscarsson; Lucy Di Silvio; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; protein adsorption; blood proteins; biomaterials; ceramics; glass-ceramics; two-dimensional gel electrophoresis; protein orientation; silicon wafers; atomic force microscopy; bone cell adhesion; divalent cation dependency; Kemi; Chemistry; Kemi;

   Sammanfattning : The objective of this thesis was to investigate and characterize the interaction between blood proteins and different surfaces with emphasis on protein adsorption to bioceramics and model surfaces. Special effort was made to monitor the spontaneous and selective adsorption of proteins from human plasma and to examine the orientation, conformation and functional behavior of single proteins after adsorption. LÄS MER

5. 4. Parallel target selection by trinucleotide threading

   Författare :Pawel Zajac; Joakim Lundeberg; Afshin Ahmadian; Mats Nilsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; trinucleotide threading; multiplex amplification; expression profiling; microarray; generic tag; short tandem repeat; microsatellite; electrophoresis; single nucleotide polymorphism; allelotyping; 454; Pyrosequencing; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

   Sammanfattning : DNA is the code for all life. Via intermediary RNA the information encoded by the genome is relayed to proteins executing the various functions in a cell. Together, this repertoire of inherently linked biological macromolecules determines all characteristics and features of a cell. LÄS MER

7. 5. Measurement of primary DNA damage in individual cells from humans and mice using the alkaline single cell gel electrophoresis assay

   Författare :Hamid Vaghef; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical biosciences; Farmaceutisk biovetenskap; Biopharmacy; Biofarmaci; arbets- och miljömedicin; Occupational and Environmental Medicine;

   Sammanfattning : Primary DNA damage is considered to be an important step in the development of tumours and itmay also serve as a biomarker of genotoxic stress. Measurement of DNA strand breaks in peripherallymphocytes can yield useful information about the suitability of these cells for biomonitoring purposesand subsequently risk assessment. LÄS MER