Sökning: "Proteinteknik"

Hittade 5 avhandlingar innehållade ordet Proteinteknik.

  1. 1. In silico protein design for the enhancement of protein stability and function

    Författare :David A. Hueting; Per-Olof Syrén; Hjalmar Brismar; Zbynek Prokop; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Ancestral sequence reconstruction; terpene cyclases; SARS-CoV-2; Spike protein; PET; PETase; rational engineering; Proteinteknik; Förfäders sekvensrekonstruktion; Terpencyklas; SARS-CoV-2; Spike protein; PET; PETas; rationell proteinteknik; Kemi; Chemistry;

    Sammanfattning : Enzymes are natures catalysts that increase the rate of a chemical reaction. The increased rate of a reaction is required to be able to sustain life. Despite the huge impact of enzymes, they are not perfect catalysts. LÄS MER

  3. 2. Identification and characterisation of chitin and cellulose synthases in oomycetes : New tools for biochemical studies and structure determination

    Författare :Stefan Klinter; Vincent Bulone; Lauren S. McKee; Mirjam Czjzek; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; biochemical characterisation; cellulose synthase; chitin synthase; in vitro activity assay; microtubule-interacting and trafficking domain; nanoparticle; oomycetes; phylogenetic analysis; yeast engineering; biokemisk karakterisering; cellulosasyntas; kitinsyntas; in vitro aktivitetsanalys; mikrotubulus-interagerande och -trafikerande domän; nanopartikel; oomyceter; fylogenetisk analys; jäst proteinteknik; Bioteknologi; Biotechnology;

    Sammanfattning : Despite resembling ‘true’ fungi in terms of morphological features, oo­mycetes form a distinct eukaryotic lineage of filamentous microorganisms that belongs to the stramenopiles, a group of protists also comprising the closely-related brown algae and diatoms. Many oomycetes are devastating pathogens of plants and animals, globally causing significant economic los­ses in the agriculture and aquaculture industries, and posing considerable environmental damage to natural ecosystems. LÄS MER

  4. 3. Mammalian protein expression and characterization tools for next generation biologics

    Författare :Niklas Thalén; Johan Rockberg; Kerstin Otte; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CHO; Cell line engineering; Protein engineering; GFP; Cell display; Biotechnology; Bioteknologi;

    Sammanfattning : Protein therapeutics are increasingly important for modern medicine. Novel recombinant proteins developed today can bind towards their target with high specificity and with low adverse effect. This has enabled the treatment of diseases that for a few years ago were deemed uncurable. LÄS MER

  5. 4. The ADAPT scaffold as a tool for diagnostic imaging and targeted therapy

    Författare :Emma von Witting; Sophia Hober; Inger Sandlie; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Protein G; ABD; ADAPT; affinity proteins; antibody; protein engineering; targeted therapy; nuclear medicine; molecular imaging; HER2; radionuclide therapy; Bioteknologi; Biotechnology;

    Sammanfattning : Molecular recognition, or the specific interactions between a protein and its ligand, is central to biology and a key factor for many different clinical and technical applications. Despite antibodies being only one of many different affinity proteins, it has by far been the most successful. LÄS MER

  7. 5. Exploitation of interactions with the neonatal Fc receptor to manipulate biological half-lives for therapeutic applications

    Författare :Shengze Yu; Torbjörn Gräslund; Mats Ohlin; KTH; []
    Nyckelord :Glioblastoma Multiforme GBM ; Prolacin Prl ; Prolacin receptor PrlR ; Prolactin antagonist PrlRA ; Albumin binding domain ABD ; serum albumin SA ; half-life extension; FcRn; Affibody; human growth hormone hGH ; immunoglobulin G IgG ; blocking; autoimmune disease; Bioteknologi; Biotechnology;

    Sammanfattning : Protein engineering provides powerful tools to create useful proteins with desired properties. In this thesis, rational design principles have been used for development of fusion proteins that can interact with the neonatal Fc receptor (FcRn) for potential medical applications. FcRn is widely expressed in the human body. LÄS MER