Sökning: "Albumin binding domain ABD"

Visar resultat 1 - 5 av 14 avhandlingar innehållade orden Albumin binding domain ABD.

  1. 1. Protein engineering to explore and improve affinity ligands

    Författare :Martin Linhult; KTH; []
    Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

    Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

  2. 2. Interaction engineered three-helix bundle domains for protein recovery and detection

    Författare :Tove Alm; Sophia Hober; Rainer Hahn; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ABD; Zbasic; albumin; protein engineering; phage display; staphylococcal display; inclusion bodies; refolding; proteomics; orthogonal affinity purification; Bioengineering; Bioteknik; Immunology engineering; Immunteknik;

    Sammanfattning : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. LÄS MER

  3. 3. Generation and engineering of ABD-derived affinity proteins for clinical applications

    Författare :Sarah Lindbo; Sophia Hober; Christoph Rader; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ABD; ADAPT; affinity proteins; protein engineering; radionuclide molecular imaging; HER2; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins that specifically recognize and bind to other molecules or structures are important tools in industrial and medical applications. Binding proteins engineered from small stable scaffold proteins have been utilized for several purposes due to their favorable biophysical properties, tolerance to mutagenesis, efficient tissue penetration and ease of production. LÄS MER

  4. 4. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications

    Författare :Mikael Åstrand; Sophia Hober; John Löfblom; Larry Gold; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Protein G; ERBB2; ERBB3; Directed evolution; phage display; staphylococcal display; bispecific; Biotechnology; Bioteknologi;

    Sammanfattning : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. LÄS MER

  5. 5. Exploitation of interactions with the neonatal Fc receptor to manipulate biological half-lives for therapeutic applications

    Författare :Shengze Yu; Torbjörn Gräslund; Mats Ohlin; KTH; []
    Nyckelord :Glioblastoma Multiforme GBM ; Prolacin Prl ; Prolacin receptor PrlR ; Prolactin antagonist PrlRA ; Albumin binding domain ABD ; serum albumin SA ; half-life extension; FcRn; Affibody; human growth hormone hGH ; immunoglobulin G IgG ; blocking; autoimmune disease; Biotechnology; Bioteknologi;

    Sammanfattning : Protein engineering provides powerful tools to create useful proteins with desired properties. In this thesis, rational design principles have been used for development of fusion proteins that can interact with the neonatal Fc receptor (FcRn) for potential medical applications. FcRn is widely expressed in the human body. LÄS MER