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Visar resultat 6 - 10 av 446 avhandlingar som matchar ovanstående sökkriterier.

1. 6. Evolving the Methodology for Detection of Primary DNA Damage : Development, adaptation and assessment of the single cell gel electrophoresis (comet) assay

   Författare :Erik Bivehed; Björn Hellman; Hanna Karlsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Comet assay; DNA damage; DNA strand breaks; Flash-comet; Genotoxicity; In vitro; Single cell gel electrophoresis; Polymerase assisted DNA damage assay PADDA ; TK6 cells; UCDAS; U937 cells; Toxikologi; Toxicology; Farmaceutisk vetenskap; Pharmaceutical Science;

   Sammanfattning : Deoxyribonucleic acid (DNA) is one of the most important molecules in nature. It is the fundamental carrier of evolutionary information and constitutes the genetic blueprint of all living organisms. Being the sole source of information, it is vital for the cell to transmit the correct genetic information from generation to generation. LÄS MER

3. 7. Detection of Biomolecules Using Volume-Amplified Magnetic Nanobeads

   Författare :Teresa Zardán Gómez de la Torre; Maria Strømme; Peter Svedlindh; Mikkel Fougt Hansen; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Magnetic biosensor; magnetic nanobeads; Brownian relaxation; padlock probe; rolling circle amplification; DNA detection; protein detection; Engineering Science with specialization in Nanotechnology and Functional Materials; Teknisk fysik med inriktning mot nanoteknologi och funktionella material;

   Sammanfattning : This thesis describes a new approach to biomolecular analysis, called the volume-amplified magnetic nanobead detection assay (VAM-DNA). It is a sensitive, specific magnetic bioassay that offers a potential platform for the development of low-cost, easy-to-use diagnostic devices. LÄS MER

4. 8. Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification

   Författare :Hanna Andréasson; Marie Allen; Ulf Gyllensten; Pål Nyrén; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; Clinical genetics; Klinisk genetik;

   Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER

5. 9. Replication Dynamics in the DNA Damage Response

   Författare :Petra Groth; Thomas Helleday; Geroge Iliakis; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA damage; DNA replication; homologous recombination; DNA double strand-breaks; ionizing radiation; alkylating agents; NATURAL SCIENCES; NATURVETENSKAP; Genetics; Genetik; molekylärgenetik; Molecular Genetics;

   Sammanfattning : Faithful DNA replication is essential and the induction of replication stress may have profound effects on genomic integrity. This is demonstrated by the formation of DNA double strand breaks (DSBs), considered to be the most toxic DNA lesions, at stalled replication forks. LÄS MER

7. 10. Barcoded DNA Sequencing for Parallel Protein Detection

   Författare :Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

   Sammanfattning : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. LÄS MER