Sökning: "Real-time PCR"

Visar resultat 1 - 5 av 296 avhandlingar innehållade orden Real-time PCR.

1. 1. Real-time PCR and its application for protein quantification

   Författare :Kristina Lind; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

   Sammanfattning : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. LÄS MER

3. 2. Kinetics Quality Assessment for real-time PCR

   Författare :Tzachi Bar; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

   Sammanfattning : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. LÄS MER

4. 3. Gene expression profiling with real-time PCR

   Författare :Anders Ståhlberg; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; single cell biology; reverse transcription; LightUp probe; cancer diagnostics; real-time PCR; gene expression profiling; tumor biology;

   Sammanfattning : The sequence of the entire human genome is available today, and this information can be used to understand, characterize, and treat complex diseases such as cancer. Many cellular functions related to survival, growth and differentiation are reflected in the gene expression pattern, and the ability to quantify transcription levels of specific genes is, and always has been, central to research on gene function. LÄS MER

5. 4. Kinetic Outlier Detection (KOD) in real-time PCR

   Författare :Tzachi Bar; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR quality assessment; Real-time PCR; KOD; Kinetic Outliner Detection;

   Sammanfattning : .... LÄS MER

7. 5. Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification

   Författare :Hanna Andréasson; Marie Allen; Ulf Gyllensten; Pål Nyrén; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; Clinical genetics; Klinisk genetik;

   Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER