Sökning: "sensitive DNA analysis"

Visar resultat 1 - 5 av 114 avhandlingar innehållade orden sensitive DNA analysis.

  1. 1. Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification

    Författare :Hanna Andréasson; Marie Allen; Ulf Gyllensten; Pål Nyrén; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; Clinical genetics; Klinisk genetik;

    Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER

  2. 2. Mitochondrial DNA in Sensitive Forensic Analysis

    Författare :Martina Nilsson; Marie Allen; Ulf Gyllensten; Antti Sajantila; Uppsala universitet; []
    Nyckelord :Genetics; Forensic genetics; Mitochondrial DNA; HVI HVII; Nuclear DNA; Quantification; Real-time PCR; Immobilised SSO probe assay; Pyrosequencing; Discrimination power; Coding region; Mixtures; Genetik;

    Sammanfattning : Genetic profiling is commonly performed on the autosomes using multiple DNA markers. Although routine forensic DNA analysis is robust and based on reliable technologies, samples with degraded or limited amounts of DNA often fail. In these cases, the analysis of mitochondrial DNA (mtDNA) can be very valuable due to the high copy number per cell. LÄS MER

  3. 3. Sensitive Identification Tools in Forensic DNA Analysis

    Författare :Hanna Edlund; Marie Allen; Niels Morling; Uppsala universitet; []
    Nyckelord :forensic genetics; STRs; Y-chromosome; Pyrosequencing; mitochondrial DNA; skeletal remains; DNA extraction; MEDICINE; MEDICIN; Medicinsk genetik; Medical Genetics;

    Sammanfattning : DNA as forensic evidence is valuable in criminal investigations. Implementation of new, sensitive and fast technologies is an important part of forensic genetic research. This thesis aims to evaluate new sensitive methods to apply in forensic DNA analysis including analysis of old skeletal remains. LÄS MER

  4. 4. Advances in DNA Detection

    Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

  5. 5. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes

    Författare :Di Wu; Masood Kamali-Moghaddam; Ulf Landegren; Ola Söderberg; Jörg Hoheisel; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. LÄS MER