Sökning: "sensitive DNA analysis"

Visar resultat 1 - 5 av 128 avhandlingar innehållade orden sensitive DNA analysis.

1. 1. Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification

   Författare :Hanna Andréasson; Marie Allen; Ulf Gyllensten; Pål Nyrén; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; Clinical genetics; Klinisk genetik;

   Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER

3. 2. Mitochondrial DNA in Sensitive Forensic Analysis

   Författare :Martina Nilsson; Marie Allen; Ulf Gyllensten; Antti Sajantila; Uppsala universitet; []
   Nyckelord :Genetics; Forensic genetics; Mitochondrial DNA; HVI HVII; Nuclear DNA; Quantification; Real-time PCR; Immobilised SSO probe assay; Pyrosequencing; Discrimination power; Coding region; Mixtures; Genetik;

   Sammanfattning : Genetic profiling is commonly performed on the autosomes using multiple DNA markers. Although routine forensic DNA analysis is robust and based on reliable technologies, samples with degraded or limited amounts of DNA often fail. In these cases, the analysis of mitochondrial DNA (mtDNA) can be very valuable due to the high copy number per cell. LÄS MER

4. 3. Sensitive Identification Tools in Forensic DNA Analysis

   Författare :Hanna Edlund; Marie Allen; Niels Morling; Uppsala universitet; []
   Nyckelord :forensic genetics; STRs; Y-chromosome; Pyrosequencing; mitochondrial DNA; skeletal remains; DNA extraction; MEDICINE; MEDICIN; Medicinsk genetik; Medical Genetics;

   Sammanfattning : DNA as forensic evidence is valuable in criminal investigations. Implementation of new, sensitive and fast technologies is an important part of forensic genetic research. This thesis aims to evaluate new sensitive methods to apply in forensic DNA analysis including analysis of old skeletal remains. LÄS MER

5. 4. Advances in DNA Detection

   Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

7. 5. Methodology and Infrastructure for Statistical Computing in Genomics : Applications for Ancient DNA

   Författare :Kristiina Ausmees; Carl Nettelblad; Mattias Jakobsson; Flora Jay; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; statistical computing; genotype imputation; ancient DNA; deep learning; dimensionality reduction; genetic clustering; distributed computing; Scientific Computing; Beräkningsvetenskap;

   Sammanfattning : This thesis concerns the development and evaluation of computational methods for analysis of genetic data. A particular focus is on ancient DNA recovered from archaeological finds, the analysis of which has contributed to novel insights into human evolutionary and demographic history, while also introducing new challenges and the demand for specialized methods. LÄS MER