Sökning: "DNA quantification"

Visar resultat 1 - 5 av 85 avhandlingar innehållade orden DNA quantification.

  1. 1. Sensitive Forensic DNA Analysis Application of Pyrosequencing and Real-time PCR Quantification

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Hanna Andréasson; Uppsala universitet.; [2005]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik;

    Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER

  2. 2. Mitochondrial DNA in Sensitive Forensic Analysis

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Martina Nilsson; Uppsala universitet.; [2007]
    Nyckelord :Genetics; Forensic genetics; Mitochondrial DNA; HVI HVII; Nuclear DNA; Quantification; Real-time PCR; Immobilised SSO probe assay; Pyrosequencing; Discrimination power; Coding region; Mixtures; Genetik;

    Sammanfattning : Genetic profiling is commonly performed on the autosomes using multiple DNA markers. Although routine forensic DNA analysis is robust and based on reliable technologies, samples with degraded or limited amounts of DNA often fail. In these cases, the analysis of mitochondrial DNA (mtDNA) can be very valuable due to the high copy number per cell. LÄS MER

  3. 3. Digital Electrical DNA Sensing

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Maoxiang Guo; KTH.; [2019]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA detection; thiol-ene-epoxy; rolling circle amplification; digital measurement; DNA stretching; microwell-array; gold nanowires; electrical measurement; specificity; limit of detection; liquid storage; disease diagnosis; lab-on-a-chip; point-of-care.;

    Sammanfattning : Molecule detection is a workhorse in life sciences and medicine, for example in cancer diagnosis and virus and bacterial detection. DNA analysis can provide vital information about the state of a host organism and its medical and health condition. LÄS MER

  4. 4. Role of Non-Homologous End-Joining in Repair of Radiation-Induced DNA Double-Strand Breaks

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Karin Karlsson; Uppsala universitet.; [2006]
    Nyckelord :Molecular biology; DNA damage; DNA repair; DSB; NHEJ; DNA-PK; ionising radiation; high-LET; heat-labile sites; PFGE; Molekylärbiologi;

    Sammanfattning : Efficient and correct repair of DNA damage, especially DNA double-strand breaks (DSBs), is vital for the survival of individual cells and organisms. Defects in the DNA repair may lead to cell death or genomic instability and development of cancer. LÄS MER

  5. 5. Selector Technology For Multiplex DNA Analysis

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Fredrik Dahl; Uppsala universitet.; [2005]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; TECHNOLOGY Bioengineering Genetic engineering including functional genomics; TEKNIKVETENSKAP Bioteknik Genteknik inkl. funktionsgenomik;

    Sammanfattning : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. LÄS MER