Sökning: "Cryo-electron microscopy"

Visar resultat 11 - 15 av 37 avhandlingar innehållade orden Cryo-electron microscopy.

  1. 11. Nanoscopic adventures : unraveling macromolecular complexes in infectious diseases via integrative structural biology

    Författare :Kai Patric Ehrenbolger; Ronnie Berntsson; Felipe Cava; Jonas Barandun; David Drew; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Infectious diseases; E. faecalis; Microsporidia; Ribosome; Proteasome; Polar Tube; Structural Biology; Single particle Cryo-Electron Microscopy;

    Sammanfattning : This thesis focuses on understanding the underlying molecular mechanisms of infectious diseases, which claim nearly 9 million lives annually. The research centers on critical analysis of pathogen mechanisms and drug resistance. I have mainly focused on two clades of pathogens: Enterococcus faecalis and microsporidia. E. LÄS MER

  3. 12. Protein structure dynamics and interplay : by single-particle electron microscopy

    Författare :Hans Elmlund; Hans Hebert; José Maria Carazo; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biophysics; biochemistry; molecular; Biophysical chemistry; Biofysikalisk kemi;

    Sammanfattning : Single-particle cryo-electron microscopy (cryo-EM) is a method capable of obtaining information about the structural organization and dynamics of large macromolecular assemblies. In the late nineties, the method was suggested to have the potential of generating “atomic resolution” reconstructions of particles above a certain mass. LÄS MER

  4. 13. Structural studies of microbubbles and molecular chaperones using transmission electron microscopy

    Författare :Johan Härmark; Philip JB Köck; Hans Hebert; Reinhard Rachel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transmission electron microscopy; Contrast agent; Microbubble; Polyvinyl alcohol; Single particle analysis; Heat shock protein; Molecular chaperone; Technology and Health; Teknik och hälsa;

    Sammanfattning : Ultrasound contrast agents (CAs) are typically used in clinic for perfusion studies (blood flow through a specific region) and border delineating (differentiate borders between tissue structures) during cardiac imaging. The CAs used during ultrasound imaging usually consist of gas filled microbubbles (MBs) (diameter 1-5 μm) that are injected intravenously into the circulatory system. LÄS MER

  5. 14. Insights into structure and dynamics of the AAA+ motor of magnesium chelatase

    Författare :Joakim Lundqvist; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioinformatik; biomathematics biometrics; Bioinformatics; medical informatics; Rhodobacter capsulatus; Tetrapyrrole; Mutants; Magnesium chelatase; Cryo-Electron microscopy single-particle 3D reconstruction; Chlorophyll biosynthesis; AAA proteins; ATPase; medicinsk informatik; biomatematik; Molecular biophysics; Molekylär biofysik; Natural science; Naturvetenskap;

    Sammanfattning : The insertion of Mg2+ into protoporphyrin IX represents the first committed step in the chlorophyll and bacteriochlorophyll biosynthetic pathways. The reaction is catalyzed by the multisubunit enzyme Mg-chelatase, which consists of three subunits, known as I (molecular weight ~40 kDa), D (~70 kDa), and H (~140 kDa). LÄS MER

  7. 15. Reverse structural biology: Cellular processes uncovered by electron microscopy

    Författare :Jacob Croft; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; flagella; nuclear envelope budding; TAILS; cryo-EM; microtubule inner protein;

    Sammanfattning : This thesis contains two projects, both using electron microscopy as the primary technique. In the first project, nuclear envelope budding (NEB) is investigated as an alternate method of transport across the nuclear envelope. LÄS MER