Sökning: "David Drew"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden David Drew.

  1. 1. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli

    Författare :David Drew; Jan-Willem de Gier; Gunnar von Heijne; Bert Poolman; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; GFP; membrane protein; topology; overexpression; high-throughput; Biochemistry; Biokemi;

    Sammanfattning : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. LÄS MER

  3. 2. Determining Ligand- and Lipid- Interactions of SLC Transporters

    Författare :Yurie Chatzikyriakidou; David Drew; Robert Fredriksson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Solute carrier transporter; nucleotide-sugar transporter; SLC35; sodium proton exchanger; SLC9B2; thermal stability assay; membrane transport; Synaptic vesicle protein 2; Biochemistry; biokemi;

    Sammanfattning : Solute carrier transporters (SLCs) mediate the inter- and intra- cellular trafficking of a plethora of substrates and are essential to cell homeostasis. Despite their importance to human physiology and their potential as therapeutic targets, many SLCs are considered orphans as the physiological substrate has not been experimentally determined. LÄS MER

  4. 3. Nanoscopic adventures : unraveling macromolecular complexes in infectious diseases via integrative structural biology

    Författare :Kai Patric Ehrenbolger; Ronnie Berntsson; Felipe Cava; Jonas Barandun; David Drew; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Infectious diseases; E. faecalis; Microsporidia; Ribosome; Proteasome; Polar Tube; Structural Biology; Single particle Cryo-Electron Microscopy;

    Sammanfattning : This thesis focuses on understanding the underlying molecular mechanisms of infectious diseases, which claim nearly 9 million lives annually. The research centers on critical analysis of pathogen mechanisms and drug resistance. I have mainly focused on two clades of pathogens: Enterococcus faecalis and microsporidia. E. LÄS MER

  5. 4. Solute Carriers in Metabolism : Regulation of known and putative solute carriers in the central nervous system

    Författare :Emilia Lekholm; Robert Fredriksson; Helgi B. Schiöth; David Drew; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Solute Carriers; transporter; amino acid starvation; glucose metabolism; MFS; SV2; Medical Science; Medicinsk vetenskap;

    Sammanfattning : Solute carriers (SLCs) are membrane-bound transporter proteins, important for nutrient, ion, drug and metabolite transport across membranes. A quarter of the human genome codes for membrane-bound proteins, and SLCs make up the largest group of transporter proteins. LÄS MER

  7. 5. The molecular basis for substrate recognition and gating in sugar transporters

    Författare :Sarah McComas; David Drew; Philip Biggin; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; membrane transport; sugar transporter; simulations; lipids; antimalarial drugs; biokemi; Biochemistry;

    Sammanfattning : Sugar is a vital sustenance for most forms of life. For a cell to take up sugar, specialized transport proteins embedded into the membrane bilayer known as sugar porters, are required. LÄS MER