Sökning: "trans-stilbene oxide"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden trans-stilbene oxide.

1. 1. Characterization of Epoxide Hydrolases from Yeast and Potato

   Författare :Lisa Tronstad-Elfström; Mikael Widersten; Michael Arand; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Epoxide hydrolase; Catalytic residues; Active site; trans-stilbene oxide; Rapid kinetics; Active site tyrosyls; Enzyme mechanism; Lewis acid; X-ray crystallography; Substrate specificity; Unidentified ORF; α β hydrolase fold; Saccharomyces cerevisiae; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : Epoxides are three-membered cyclic ethers formed in the metabolism of foreign substances and as endogenous metabolites. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to yield the corresponding diols. LÄS MER

3. 2. Exploring Selectivity and Hysteresis : Kinetic Studies on a Potato Epoxide Hydrolase

   Författare :Diana Lindberg; Mikael Widersten; Gunnar Johansson; Richard N. Armstrong; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Epoxide hydrolase; regioselectivity; selectivity; hysteresis; conformational changes; cooperativity; deep eutectic solvents; methylstyrene oxide; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : The kinetic mechanism of an α/β hydrolase fold epoxide hydrolase from potato, StEH1, has been studied with the aims of explaining the underlying causes for enantio- and regioselectivity, both being important for product purity. Further effort has been laid upon understanding the causes of a hysteretic behavior discovered in the measurements leading to Paper I. LÄS MER

4. 3. Microsomal epoxide hydrolase : characterization, induction, and in vitro modulation, of the catalytic activity

   Författare :Janeric Seidegård; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Microsomal epoxide hydrolase has been characterized, mainly in the fiver and lung of the rat.A reported radiometric assay using 3H-styrene 7,8-oxide as substrate has been modified by purifying the substrate before use, decreasing the incubation volume, and prolonging the incubation time. LÄS MER

5. 4. Isolation and characterization of glutathione S-transferases from various animal sources

   Författare :Claes Guthenberg; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : In the present investigation several forms of glutathione S-transferase were found in preparations of rat liver, rat lung, adult human liver, fetal human liver and whole earthworms. Human placenta, however, appeared to contain only one form of glutathione S-transferase. LÄS MER

7. 5. Evolutionary Analysis and Posttranslational Chemical Modifications in Protein Redesign : A Study on Mu Class Glutathione Transferases

   Författare :Ylva Ivarsson; Bengt Mannervik; Gun Stenberg; Richard N. Armstrong; Uppsala universitet; []
   Nyckelord :Biochemistry; glutathione transferase; evolutionary analysis; positive selection; epoxide; stereoselectivity; protein modification; Biokemi;

   Sammanfattning : Glutathione transferases (GSTs) constitute a family of multifarious enzymes that conjugate glutathione (GSH) with a wide range of electrophiles. GSTs are grouped into different classes based on protein sequence similarities. Despite high sequence identities between GSTs of the same class they often display different substrate specificites. LÄS MER