Sökning: "Epoxide hydrolase"

Visar resultat 1 - 5 av 28 avhandlingar innehållade orden Epoxide hydrolase.

  1. 1. Microsomal epoxide hydrolase : characterization, induction, and in vitro modulation, of the catalytic activity

    Författare :Janeric Seidegård; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Microsomal epoxide hydrolase has been characterized, mainly in the fiver and lung of the rat.A reported radiometric assay using 3H-styrene 7,8-oxide as substrate has been modified by purifying the substrate before use, decreasing the incubation volume, and prolonging the incubation time. LÄS MER

  3. 2. Towards Understanding of Selectivity & Enantioconvergence of an Epoxide Hydrolase

    Författare :Åsa Janfalk Carlsson; Mikael Widersten; Uwe T. Bornscheuer; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Epoxide hydrolase; Epoxide; Enantioselectivity; Regioselectivity; Enantioconvergence; Crystal structures; Biocatalysis; Immobilization; Transient kinetics; CASTing; Directed evolution; Biokemi; Biochemistry;

    Sammanfattning : Epoxide hydrolase I from Solanum tuberosum (StEH1) and isolated variants thereof has been studied for mapping structure-function relationships with the ultimate goal of being able to in silico predict modifications needed for a certain activity or selectivity. To solve this, directed evoultion using CASTing and an ISM approach was applied to improve selectivity towards either of the enantiomeric product diols from (2,3-epoxypropyl)benzene (1). LÄS MER

  4. 3. Cytosolic epoxide hydrolase in murine liver : purification, characterization, regulation, species distribution and comparison to microsomal epoxide hydrolase

    Författare :Johan Meijer; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : .... LÄS MER

  5. 4. Biochemical Studies on a Plant Epoxide Hydrolase : Discovery of a Proton Entry and Exit Pathway and the Use of In vitro Evolution to Shift Enantioselectivity

    Författare :Ann Gurell; Mikael Widersten; Bengt Mannervik; Dick Janssen; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; epoxide hydrolase; enantioselectivity; in vitro evolution; proton wire; epoxides; selectivity; CASTing; structure-function; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : The work leading to this thesis has provided additional information and novel knowledge concerning structure-function relationship in the potato epoxide hydrolase. Epoxide hydrolases are enzymes catalyzing the hydrolysis of epoxides to yield the corresponding vicinal diols. LÄS MER

  7. 5. Characterization of Epoxide Hydrolases from Yeast and Potato

    Författare :Lisa Tronstad-Elfström; Mikael Widersten; Michael Arand; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Epoxide hydrolase; Catalytic residues; Active site; trans-stilbene oxide; Rapid kinetics; Active site tyrosyls; Enzyme mechanism; Lewis acid; X-ray crystallography; Substrate specificity; Unidentified ORF; α β hydrolase fold; Saccharomyces cerevisiae; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : Epoxides are three-membered cyclic ethers formed in the metabolism of foreign substances and as endogenous metabolites. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to yield the corresponding diols. LÄS MER