Sökning: "substrate interaction"

Visar resultat 6 - 10 av 301 avhandlingar innehållade orden substrate interaction.

  1. 6. Enzyme substrate solvent interactions : a case study on serine hydrolases

    Författare :Linda Fransson; Karl Hult; Jürgen Pleiss; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; esterase; specificity; Bioengineering; Bioteknik;

    Sammanfattning : Reaction rates and selectivities were measured for transacylation of fatty acid esters in solvents catalysed by Candida antarctica lipase B and by cutinase from Humicola insolens. With these enzymes classical water-based enzymology can be expanded to many different solvents allowing large variations in interaction energies between the enzymes, the substrates and the surrounding. LÄS MER

  3. 7. Exploring small heat shock protein chaperones by crosslinking mass spectrometry

    Författare :Wietske Lambert; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cross-linking; mass spectrometry; protein-protein interactions; Hsp21; BS3; DTSSP; MALDI-TOF TOF; client protein; substrate; aggregation; unfolded protein; chaperone; Small heat shock protein; crosslinking;

    Sammanfattning : Together with other molecular chaperones, small heat shock proteins are key components of the protein quality control system, which is comprised of several hundred proteins and acts to maintain proteome homeostasis in the cell. Small heat shock proteins bind unfolding proteins at an early stage, to prevent these from further unfolding and aggregating. LÄS MER

  4. 8. Distal to Proximal—Functional Coupling in RNase P RNA-mediated Catalysis

    Författare :Shiying Wu; Leif A. Kirsebom; Britt-Marie Sjöber; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribozyme; RNase P; Induced fit model; tRNA progressing; Substrate interaction;

    Sammanfattning : RNase P is a ubiquitous ribonuclease responsible for removing the 5’ leader of tRNA precursor. Bacterial RNase P contains one RNA (RPR) and one protein (RPP) subunit. However, the number of protein variants depends on the origin. The RNA subunit is the catalytic subunit that in vitro cleaves its substrate with and without the protein subunit. LÄS MER

  5. 9. Interaction Studies of Secreted Aspartic Proteases (Saps) from Candida albicans : Application for Drug Discovery

    Författare :Dan Backman; Helena Danielson; Birgitta Tomkinson; Gunnar Henriksson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; SPR Biosensor; Secreted aspartic proteases; Candida albicans; interaction kinetics; drug discovery; protease inhibitor; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : This thesis is focused on enzymatic studies of the secreted aspartic proteases (Saps) from Candida albicans as a tool for discovery of anti-candida drugs. C. albicans causes infections in a number of different locations, which differ widely in the protein substrates available and pH. Since C. LÄS MER

  7. 10. Metal ion cooperativity in Escherichia coli RNase P RNA

    Författare :Mathias Brännvall; Britt-Marie Sjöberg; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; RNase P; divalent metal ions; substrate interaction; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; mikrobiologi; Microbiology;

    Sammanfattning : RNase P is an essential ribonuclease responsible for removal of the 5’ leader of tRNA precursors. Bacterial RNase P consists of an RNA subunit and a small basic protein. The catalytic activity is associated with the RNA subunit, i.e. LÄS MER