Sökning: "Ribozyme"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet Ribozyme.

  1. 1. Investigation of RNase P active site residues and catalytic domain interaction

    Författare :Guanzhong Mao; Leif Kirsebom; Roland Hartmann; Britt-marie Sjöberg; Anders Byström; Anthony Forster; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNase P; Ribozyme; PRORP; Induced fit model; RNase P-pre-tRNA interaction; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : RNase P is an essential endoribonuclease responsible for the maturation of the tRNA 5’end. The RNase P family encompasses the ribozyme based, RNase P RNP, and proteinaceous RNase P (PRORP). The ribozyme based RNase P is widely distributed in most species while PRORP has so far mainly been found in some eukaryotic cells. LÄS MER

  3. 2. Versatile and Antique World of RNA : The Simplicity of RNA Mediated Catalysis

    Författare :Ema Kikovska; Leif Kirsebom; Venkat Gopalan; Uppsala universitet; []
    Nyckelord :Microbiology; RNA Catalysis; RNase P; Metal ions; tRNA Processing; Ribozyme; Mikrobiologi;

    Sammanfattning : RNA is the only biological molecule that can function both as a repository of information and as a catalyst. This, together with the ability to self-replicate, led to recognition of RNA as ‘prelude to life’.My work highlights some of the important features of RNA as a catalyst, exemplified by RNase P. LÄS MER

  4. 3. Distal to Proximal—Functional Coupling in RNase P RNA-mediated Catalysis

    Författare :Shiying Wu; Leif A. Kirsebom; Britt-Marie Sjöber; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribozyme; RNase P; Induced fit model; tRNA progressing; Substrate interaction;

    Sammanfattning : RNase P is a ubiquitous ribonuclease responsible for removing the 5’ leader of tRNA precursor. Bacterial RNase P contains one RNA (RPR) and one protein (RPP) subunit. However, the number of protein variants depends on the origin. The RNA subunit is the catalytic subunit that in vitro cleaves its substrate with and without the protein subunit. LÄS MER

  5. 4. Preclinical studies of ribozyme-mediated gene therapy for HIV-1

    Författare :Catharina Maijgren Steffensson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :AIDS; antiretroviral; cis-cleavage; gene therapy; HAART; hairpin; hammerhead; HIV-1; in vitro; in vivo; ribozyme; RNA;

    Sammanfattning : Approximately 40 million people are infected with HIV-1 and AIDS is the number one cause of death in Africa as well as, the fourth leading cause of death globally. Patients in developed countries generally receive highly active antiretroviral therapy (HAART), even though the drugs are expensive and sometimes cause side-effects and treatment failure. LÄS MER

  7. 5. Towards ribozyme-mediated gene therapy of HIV-1 infections

    Författare :Graham Hotchkiss; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Ribozymes; HIV-1; Telomere length; HAART; Gene Therapy;

    Sammanfattning : HIV-1 is the causative agent in AIDS, the world's fourth most frequent cause of death. HIV is a retrovirus that primarily infects macrophages and T helper cells - required for a proper immune response. LÄS MER