Sökning: "real-time quantitative PCR"

Visar resultat 31 - 35 av 102 avhandlingar innehållade orden real-time quantitative PCR.

1. 31. Aetiology in community-acquired pneumonia

   Författare :Niclas Johansson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Background: Although community-acquired pneumonia (CAP) is a common and well-known disease, its microbial aetiology is still not well characterized. During the past few years nucleic acid detection using real-time polymerase chain reaction (PCR) has been developed for detection of many bacterial and viral pathogens causing respiratory tract infections. LÄS MER

3. 32. Nitrogen fixation among marine bacterioplankton

   Författare :Kjärstin H. Boström; Lasse Riemann; Åke Hagström; Grieg Steward; Högskolan i Kalmar; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bacterioplankton; Nitrogen fixation; Bakterioplankton; Kvävefixering; Cyanobakterier; Kvävefixerande bakterier; Microbiology; Mikrobiologi; Mikrobiologi; Microbiology;

   Sammanfattning : While bacterioplankton indisputably control vital biogeochemical paths in the cycling of carbon and nutrients in the world’s oceans, our knowledge about the functional and genetic diversity of bacterioplankton communities is negligible. In this thesis, molecular and more traditional microbiological methods were used to study the specific function of N2-fixation and in a general sense diversity of marine bacterioplankton species. LÄS MER

4. 33. Delection mapping of human 3p in major epithelial types of cancer and fine localization of candidate tumor suppressor genes

   Författare :Jian Liu; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Tumor suppressor gene; epithelial carcinoma; human chromosome 3p;

   Sammanfattning : Allele loss and deletion mapping using microsatellite markers and the detection of homozygous deletions represented until now the most powerful method to localize potential TSGs. Loss of heterozygosity (LOH) involving several chromosome 3p regions accompanied by chromosome 3p deletions are detected in almost 100% of renal cell carcinoma (RCC), small (SCLCs) and more than 90% of non-small (NSCLC) cell lung cancers. LÄS MER

5. 34. Expression profiling using manifold supports

   Författare :Anette Hagberg; Joakim Lundeberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; expression; minimal residual disease MRD ; chronic myeloid leukemia CML ; recombinant human erythropoietin r-HuEpo ; anemia; insulin dependent diabetes mellitus IDDM ; transplantation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Sammanfattning : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. LÄS MER

7. 35. Respiratory NAD(P)H dehydrogenases of plants - Gene identity and expression in response to light and cold

   Författare :Staffan Svensson; Biologiska institutionen; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; morfologi; phytogeography; chemotaxonomy. Physiology of nonvascular plants; Systematisk botanik; taxonomi; morphology; taxonomy; Systematic botany; Växtgenetik; Plant genetics; plant mitochondria; NADH dehydrogenases; light dependence; mRNA quantification; leaf respiration; cold treatment; kemotaxonomi. Växters fysiologi inte kärlväxter .;

   Sammanfattning : The respiratory chain of plants contains class 2 NAD(P)H dehydrogenases, not present in animals. The functional roles of these enzymes have been elusive for a long time. Two cDNAs, homologous to class 2 NADH dehydrogenase genes of yeast and E. coli, were isolated from potato leaves. LÄS MER