Sökning: "mRNA quantification"

Visar resultat 1 - 5 av 42 avhandlingar innehållade orden mRNA quantification.

  1. 1. Genital human papillomaviruses. Studies of their occurrence, type spectrum and expression

    Författare :Ola Forslund; Malmö Klinisk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; mRNA expression; microwell; HPV 70; type determination; CIN; quantification; urine; multifocal infections; hybridization; PCR; HPV; human papillomavirus; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : The occurrence of genital human papillomavirus infection was determined in different categories of patients by means of PCR. In high proportion of young women attending an adolescent clinic, the presence of HPV DNA was demonstrated in simultaneously urethral and cervical samples, suggesting that genital HPV infections are often multifocal. LÄS MER

  2. 2. High-throughput protein analysis using mass spectrometry-based methods

    Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

  3. 3. Development of novel molecular methods for Campylobacter species and for Helicobacter pylori

    Författare :Kristina Blom; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Campylobacter; Helicobacter pylori; PCR; mRNA; hybridization; flow cytometry; HpaA; urease; NAP;

    Sammanfattning : Background and Aim: Different Campylobacter species and Helicobacter pylori are common causes of gastrointestinal infections that may result in diarrhea and chronic gastritis, respectively. The overall aim of this thesis was to develop novel methods for identification, differentiation and characterization of these bacteria. LÄS MER

  4. 4. Respiratory NAD(P)H dehydrogenases of plants - Gene identity and expression in response to light and cold

    Författare :Staffan Svensson; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; morfologi; phytogeography; chemotaxonomy. Physiology of nonvascular plants; Systematisk botanik; taxonomi; morphology; taxonomy; Systematic botany; Växtgenetik; Plant genetics; plant mitochondria; NADH dehydrogenases; light dependence; mRNA quantification; leaf respiration; cold treatment; kemotaxonomi. Växters fysiologi inte kärlväxter .;

    Sammanfattning : The respiratory chain of plants contains class 2 NAD(P)H dehydrogenases, not present in animals. The functional roles of these enzymes have been elusive for a long time. Two cDNAs, homologous to class 2 NADH dehydrogenase genes of yeast and E. coli, were isolated from potato leaves. LÄS MER

  5. 5. Quantification of gene expression in single cells

    Författare :Martin Bengtsson; Islet cell physiology; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicin människa och djur ; Medicine human and vertebrates ; Biomedical sciences; Biomedicinska vetenskaper; quantitative gene expression analysis; single cell PCR; islets of Langerhans;

    Sammanfattning : Studies of apparently homogeneous cell populations and single cells often give highly divergent results. Cells exhibit varying responsiveness to stimuli and gene expression and they are in many aspects stochastic and unpredictable. We have developed a method to measure gene expression quantitatively in individual cells with real-time RT-PCR. LÄS MER