Sökning: "reaction specificity"

Visar resultat 36 - 40 av 130 avhandlingar innehållade orden reaction specificity.

1. 36. Deoxyribonucleoside kinases in bacteria, plants and humans

   Författare :Anders Ranegaard Clausen; Biologiska institutionen; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; anti-viral drugs; gene therapy; cancer; evolution; nucleoside analog; deoxyribonucleoside kinase; thymidine kinase;

   Sammanfattning : Deoxyribonucleoside triphosphates (dNTPs) are the building blocks of DNA and can be synthesized either de novo or via salvage pathways. The first reaction in the salvage pathway of dNTPs is the conversion of deoxyribonucleosides (dN) into the deoxyribonucleoside monophosphate (dNMP). LÄS MER

3. 37. Evasion and Attack: Structural Studies of a Bacterial Albumin-binding Protein and of a Cephalosporin Biosynthetic Enzyme

   Författare :Sara Lejon; Janos Hajdu; Karin Valegård; Martin Noble; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; human serum albumin; GA module; albumin-binding; Finegoldia magna; deacetylcephalosporin C acetyltransferase; cephalosporin C; antibiotic; beta-lactam; biosynthesis; Acremonium chrysogenum; X-ray crystallography; Structural biology; Strukturbiologi;

   Sammanfattning : This thesis describes the crystal structures of two proteins in the context of combatting bacterial infections. The GA module is a bacterial albumin-binding domain from a surface protein expressed by pathogenic strains of the human commensal bacterium Finegoldia magna. LÄS MER

4. 38. Genotyping RNA and DNA using padlock probes

   Författare :Dan-Oscar Antson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Sammanfattning : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. LÄS MER

5. 39. The influence of nucleotides on ribonucleotide reductase assambly in class I ribonucleotide reductase from Escherichia coli

   Författare :Pernilla Larsson Birgander; Britt-Marie Sjöberg; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Molekylärbiologi;

   Sammanfattning : The components of DNA, the deoxyribonucleotides, are produced from the components of RNA, the ribonucleotides. One single substitution is needed to convert a ribonucleotide into a deoxyribonucleotide i.e. a replacement of a hydroxyl group with a hydrogen atom. LÄS MER

7. 40. Detection of immunoglobulin heavy chain gene rearrangement with PCR for MRD analysis in lymphoproliferative disorders

   Författare :Ulf Thunberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Minimal residual disease; Immunoglobulin gene rearrangement; PCR; Leukemia; Lymphoma; Myeloma; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

   Sammanfattning : Immunoglobulin heavy chain (IGH) gene rearrangement occurs during early B-lymphocyte differentiation, assembling the different IGH gene segments to a functional gene, which can serve as a marker for study of lineage association and detection of Minimal Residual Disease (MRD) in clonal diseases deriving from B-lymphocytes or their early differentiation stages. Use of a molecular marker for the leukemic cells could help improve treatment by monitoring therapeutic efficacy, predicting relapse, and identifying very small amounts of tumour cells contaminating autografts after purging or enrichment of stem cells. LÄS MER