Sökning: "PCR"

Visar resultat 1 - 5 av 1123 avhandlingar innehållade ordet PCR.

1. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

   Författare :Rickard Knutsson; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. LÄS MER

3. 2. PCR inhibition mechanisms in forensic DNA analysis

   Författare :Maja Sidstedt; Teknisk mikrobiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

   Sammanfattning : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. LÄS MER

4. 3. Detection and Characterisation of Salmonella in Animal Feed Samples by PCR-Based Methods

   Författare :Charlotta Löfström; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Food and drink technology; Livsmedelsteknik; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; Analytisk kemi; Analytical chemistry; mykologi; virologi; bakteriologi; Mikrobiologi; validation; Microbiology; bacteriology; virology; mycology; Salmonella; ribotyping; detection; animal feed; randomly amplified polymorphic DNA; pulsed-field gel electrophorsis; pre-PCR processing; PCR; polymerase chain reaction; genotyping;

   Sammanfattning : Animal feed is a recognised source of Salmonella enterica for farm livestock and may also indirectly cause infection in people consuming foods of animal origin. It is therefore important to have rapid, reproducible and specific methods for the detection of Salmonella in feed, and for the characterisation of strains for further epidemiological investigations or to trace the source of contamination in a production facility. LÄS MER

5. 4. DNA Analysis of PCR-Inhibitory Forensic Samples

   Författare :Johannes Hedman; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amylase; statistical modelling; principal components analysis; PCR inhibitors; PCR inhibition; forensic DNA analysis; DNA polymerase blend; DNA polymerase; crime scene samples;

   Sammanfattning : DNA evidence, linking perpetrators to crime scenes, is central to many legal proceedings. However, crime scene samples often contain extraneous substances that may interfere with the PCR-based forensic analysis, resulting in partial or negative DNA profiles. Extensive DNA purification may remove inhibitors, but involves the risk of DNA loss. LÄS MER

7. 5. Assesing performance of PCR analysis using Bayesian modelling

   Författare :Ronny Hedell; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
   Nyckelord :NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; Bayesian; Hierarchical model; PCR; Low-template DNA; Bacillus anthracis; Sensitivity; Forensic science; Forensic science;

   Sammanfattning : Different analytical methods are available to analyse the DNA samples from crime scenes or to screen samples for the presence of some target bacterium. For this purpose a method known as Polymerase Chain Reaction (PCR) can often be used. LÄS MER