Sökning: "microscopy automation"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden microscopy automation.

1. 1. Automating STED microscopy for functional and structural live-cell imaging

   Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

   Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

3. 2. Data-driven microscopy: placing high-fidelity data in a population-wide context

   Författare :Oscar André; Infektionsmedicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Data-driven microscopy; Event-driven microscopy; high-content screening; automation; image analysis; data analysis; cell heterogeneity; host-pathogen interactions; Streptococcus pyogenes GAS ; SARS-CoV-2;

   Sammanfattning : Mikroskopi är idag ett fundamentalt verktyg inom forskning, där det tillåter oss att skåda in och utforska våra prover i hög detalj. Mycket utav utvecklingen av nya mikroskopimetoder har strävat efter att öka den detaljnivå vi kan uppnå. LÄS MER

4. 3. Automated super-resolution microscopy for high-throughput imaging

   Författare :Xavier Casas Moreno; Ilaria Testa; Florian Jug; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RESOLFT; throughput; microscopy automation; nanoscopy; Biologisk fysik; Biological Physics;

   Sammanfattning : Fluorescence microscopes enable the visualization of biological samples with high contrast by labeling specific structures with fluorescent molecules. However, the spatial resolution of widely used microscopy techniques, such as widefield and confocal microscopy, is limited by the size of a focused spot of light, which roughly corresponds to half the wavelength of the illumination. LÄS MER

5. 4. Adapting Deep Learning for Microscopy: Interaction, Application, and Validation

   Författare :Ankit Gupta; Carolina Wählby; Ida-Maria Sintorn; Ola Spjuth; Andreas Hellander; Philip Kollmannsberger; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Deep Learning; Microscopy; Human-in-the-Loop; Semi-Supervised Learning; Application-Specific Analysis; Image Classification; Image-to-Image Translation; Template Matching; Computerized Image Processing; Datoriserad bildbehandling;

   Sammanfattning : Microscopy is an integral technique in biology to study the fundamental components of life visually. Digital microscopy and automation have enabled biologists to conduct faster and larger-scale experiments with a sharp increase in the data generated. LÄS MER

7. 5. Single Molecule Detection : Microfluidic Automation and Digital Quantification

   Författare :Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

   Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER