Sökning: "Uwe T. Bornscheuer"

Hittade 2 avhandlingar innehållade orden Uwe T. Bornscheuer.

1. 1. Towards Understanding of Selectivity & Enantioconvergence of an Epoxide Hydrolase

   Författare :Åsa Janfalk Carlsson; Mikael Widersten; Uwe T. Bornscheuer; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Epoxide hydrolase; Epoxide; Enantioselectivity; Regioselectivity; Enantioconvergence; Crystal structures; Biocatalysis; Immobilization; Transient kinetics; CASTing; Directed evolution; Biokemi; Biochemistry;

   Sammanfattning : Epoxide hydrolase I from Solanum tuberosum (StEH1) and isolated variants thereof has been studied for mapping structure-function relationships with the ultimate goal of being able to in silico predict modifications needed for a certain activity or selectivity. To solve this, directed evoultion using CASTing and an ISM approach was applied to improve selectivity towards either of the enantiomeric product diols from (2,3-epoxypropyl)benzene (1). LÄS MER

3. 2. Protein Engineering of Candida antarctica Lipase A : Enhancing Enzyme Properties by Evolutionary and Semi-Rational Methods

   Författare :Anders G. Sandström; Jan-Erling Bäckvall; Uwe T. Bornscheuer; Stockholms universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; protein engineering; directed evolution; kinetic resolution; structural biology; Enzyme engineering; Enzymteknik; organisk kemi; Organic Chemistry;

   Sammanfattning : Enzymes are gaining increasing importance as catalysts for selective transformations in organic synthetic chemistry. The engineering and design of enzymes is a developing, growing research field that is employed in biocatalysis. LÄS MER