Sökning: "chromatography matrices"

Visar resultat 6 - 10 av 56 avhandlingar innehållade orden chromatography matrices.

  1. 6. Analysis of drugs and proteins in biological matrices, using different types of sample preparations and mass spectrometry

    Författare :Aljona Saleh; Ingrid Granelli; Jonas Bergquist; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioanalysis; drugs; proteins; liquid chromatography; two-dimensional liquid chromatography; mass spectrometry; Analytical Chemistry; analytisk kemi;

    Sammanfattning : This thesis describes bioanalysis of small and large molecules in biological matrices and includes screening of illegal drugs in urine with high resolution mass spectrometer, bioanalysis of MTH1 inhibitors in mice plasma and quantification of proteins in plasma and cell lysates.Screening of illegal drugs was based on high resolving power mass spectrometer (QTOF) and the results were compared to immunoassays. LÄS MER

  3. 7. Polymer Displacement and Supermacroporous Cryogels - Two New Tools in Chromatographic Separation of Biomolecules and Bioparticles

    Författare :Pär Arvidsson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biopolymers; Polymerteknik; Biochemical technology; Polymer technology; IMAC; dye-affinity; affinity chromatography; viable cells; cell chromatography; non-clarified cell homogenate; cryogel; Polymer displacement; supermacroporous; Biokemisk teknik;

    Sammanfattning : Production of protein is often accompanied with difficulties due to the large amounts of different biological material resulting from fermentation. This makes purification necessary and affinity chromatography is usually involved as being biospecific, although suffering from limitations as low recovery due to ineffective elution and inability to handle particulate materials. LÄS MER

  4. 8. Artificial receptors: New opportunities for the exploitation of molecularly imprinted polymers

    Författare :Lei Ye; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Radioimmunoassay; Microsphere; Molecular imprinting; Drug determination; Cross-linking polymerisation; Combinatorial library; Chromatography; Chiral separation; Affinity adsorbent; Biosensor; Receptor; Biochemistry; Metabolism; Biokemi; metabolism; Biotechnology; Bioteknik;

    Sammanfattning : Molecular imprinting, method for creating artificial receptors that are mimics of biological antibodies and receptors, is based on the concept of creating substrate-specific recognition sites in polymeric matrices by means of template polymerisation. The polymeric receptors produced display favourable binding characteristics, such as high affinity and specificity towards the substrate molecules. LÄS MER

  5. 9. Long-term Properties of Sustainable Polymeric Materials : Mechanical Recycling and Use of Renewable Resources

    Författare :Emma Strömberg; Sigbritt Karlsson; Amparo Ribes-Greus; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Sustainable materials; mechanical recycling; polyolefin; reprocessing; accelerated ageing; chromatography; biofilm; microenvironment chamber; environmental degradation; biocomposite; nanocomposite; Polymer chemistry; Polymerkemi;

    Sammanfattning : New strategies for management of the accumulating amounts of plastic waste are required, to achieve a sustainable development in terms of material production and use. After service life, the materials should be recovered and recycled efficiently to provide a valuable resource for future applications. LÄS MER

  7. 10. Analysis of Complex Biological Samples using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

    Författare :Margareta Ramström; Jonas Bergquist; Per Håkansson; Karin Markides; Carol Robinson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; mass spectrometry; liquid chromatography; protein; proteomics; peptide; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyotrophic lateral sclerosis; electrospray ionization; electron capture dissociation; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Studies of protein and peptide expression are vital in order to understand complex biological systems. As demonstrated in this thesis, on-line packed capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) is a useful analytical tool for such studies. LÄS MER