Sökning: "calcium-binding proteins"

Visar resultat 21 - 25 av 50 avhandlingar innehållade orden calcium-binding proteins.

1. 21. The GDNF-receptor GFR-alpha-1 : neural expression patterns and regulation in stroke

   Författare :Arezou Sarabi; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :G DNF; GFR-alpha-1 Strok; . Ischemia; receptors; calcium-binding proteins; gamma- aminobutyric acid; glutamic acid decarboxylase; dopamine; Parkinson s disease;

   Sammanfattning : Background: Neurotrophic factors in the mammalian nervous system are important for development and survival of neurons and for maintenance and plasticity of synaptic connections. Additionally, they have been shown to prevent neurodegeneration after damage to the central nervous system (CNS). LÄS MER

3. 22. Fecal calprotectin in children with special reference to inflammatory bowel disease

   Författare :Ulrika Lorentzon Fagerberg; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Biological markers; calcium-binding proteins; Leukocyte L1 Antigen Complex; calprotectin; acute- phase proteins; serum amyloid A; C-reactive protein; ELISA; feces; blood; children; adolescent; colitis; inflammatory bowel disease; colonoscopy; reference values; diagnosis.;

   Sammanfattning : This thesis aims to study the clinical usefulness of fecal calprotectin as a noninvasive marker of colonic inflammation in children with suspected or confirmed chronic inflammatory bowel disease (IBD). Calprotectin, a calcium-binding protein predominantly expressed in neutrophils, is stable in feces for several days, and can be measured by an enzyme-linked immunosorbent assay. LÄS MER

4. 23. Protein Complexity via Non-Native States: Binding, Stability, and Structural Studies of Calbindin D9k and HAMLET (Human alpha-lactalbumin Made LEthal to Tumor cells)

   Författare :Jonas Fast; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; apoptosis; oleic acid; HAMLET; kinetic trap alpha-lactalbumin; calbindin; calcium; Metabolism; Biokemi; metabolism;

   Sammanfattning : Using optical spectroscopy, nuclear magnetic resonance (NMR), and differential scanning Calorimetry (DSC), I have studied two different calcium binding proteins that can form kinetically trapped altered states. Calcium is very important in numerous biological processes such as blood coagulation, signal transduction, muscle contraction and bone formation. LÄS MER

5. 24. Shaking the EF-Hand. Dynamics and Ion-Binding to Calmodulin and Calbindin D9k

   Författare :Anders Malmendal; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; magnesium; line shape; ion binding; EF-hand; cooperativity; conformational exchange; chemical shift; calmodulin; calcium; calbindin D9k; allostery; backbone dynamics; NMR; relaxation; Physical chemistry; Fysikalisk kemi;

   Sammanfattning : The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. The x-ray structure of magnesium-saturated calbindin D9k shows a magnesium ion in only one of the two ion binding sites. LÄS MER

7. 25. Engineering of small IgG binding domains for antibody labelling and purification

   Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

   Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER