Sökning: "DNA detection"

Visar resultat 21 - 25 av 446 avhandlingar innehållade orden DNA detection.

  1. 21. Engineering Surfaces of Solid-State Nanopores for Biomolecule Sensing

    Författare :Shiyu Li; Shi-Li Zhang; Michael Mayer; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; solid-state nanopores; surface engineering; DNA sensing; DNA polymerase; optical detection; lipid bilayer; hafnium oxide; electron beam induced deposition;

    Sammanfattning : Nanopores have emerged as a special class of single-molecule analytical tool that offers immense potential for sensing and characterizing biomolecules such as nucleic acids and proteins. As an alternative to biological nanopores, solid-state nanopores present remarkable versatility due to their wide-range tunability in pore geometry and dimension as well as their excellent mechanical robustness and stability. LÄS MER

  3. 22. Arrayed identification of DNA signatures

    Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

    Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

  4. 23. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    Författare :Ingrid Ziegler; Kristoffer Strålin; Paula Mölling; Magnus Rasmussen; Örebro universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Sammanfattning : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. LÄS MER

  5. 24. Exploring the multiscale 3D architecture of human chromosome contact networks

    Författare :Dolores Bernenko; Ludvig Lizana; Martin Rosvall; Petter Holme; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; community detection; DNA 3D organization; Hi-C; network science; DNA-DNA contact data;

    Sammanfattning : Cells regulate genes to coordinate essential functions allowing cells to grow, divide, specialize, and respond to stresses. While regulatory proteins are the most common way to control these genes, the DNA’s 3D structure also plays a critical role as it affects how proteins access genes and how regulatory DNA elements interact over large genomic distances. LÄS MER

  7. 25. Electric DNA arrays for determination of pathogenic Bacillus cereus

    Författare :Yanling Liu; Sven-Olof Enfors; Helena Anderson; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacillus cereus; haemolysin; non-haemolytic enterotoxin; cytotoxin K-2; cereulide; toxin-coding genes; bacterial colony; electric DNA chip; ultrasonication; DNA fragmentation; Biochemistry; Biokemi;

    Sammanfattning : Silicon-based electric chip arrays were developed for characterization of Bacillus cereus with respect to the capacity to produce toxins involved in food poisoning and foodborne infections. Bacteria of the B. cereus group contain different sets of four toxins encoded by eight genes. LÄS MER