Avancerad sökning

Hittade 3 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Automating STED microscopy for functional and structural live-cell imaging

    Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

    Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

  3. 2. Quantitative bioimaging in single cell signaling

    Författare :Kristoffer Bernhem; Hjalmar Brismar; Anita Aperia; Lena Scott; Christian Eggeling; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Super resolution imaging; fluoresence; bioimaging; cells; FRET; cluster analysis; labeling; image analysis.;

    Sammanfattning : Imaging of cellular samples has for several hundred years been a way for scientists to investigate biological systems. With the discovery of immunofluorescence labeling in the 1940’s and later genetic fluorescent protein labeling in the 1980’s the most important part in imaging, contrast and specificity, was drastically improved. LÄS MER

  4. 3. Super resolution optical imaging – image analysis, multicolor development and biological applications

    Författare :Daniel Rönnlund; Jerker Widengren; Christian Eggeling; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Stimulated emission depletion STED microscopy; nanoscopy; multicolor; image analysis; diagnostics; cancer; metastasis; Biological Physics; Biologisk fysik;

    Sammanfattning : This thesis focuses on super resolution STED optical imaging. STED provides a wealth of new informational content to the acquired images by using stimulated emission to surpass the diffraction limit in optical fluorescence microscopy. LÄS MER