Sökning: "Chinese Hamster Ovary Cell"

Visar resultat 16 - 20 av 27 avhandlingar innehållade orden Chinese Hamster Ovary Cell.

1. 16. Systems Biology of Protein Secretion in Human Cells: Multi-omics Analysis and Modeling of the Protein Secretion Process in Human Cells and its Application

   Författare :Rasool Saghaleyni; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Erythropoietin; cancer protein secretory pathway; protein secretion modeling; HEK293; protein secretion; Integrative omics analysis; genome-scale modeling;

   Sammanfattning : Since the emergence of modern biotechnology, the production of recombinant pharmaceutical proteins has been an expanding field with high demand from industry. Pharmaceutical proteins have constituted the majority of top-selling drugs in the pharma industry during recent years. LÄS MER

3. 17. Studies on O-glycosylation of mucin-type proteins and their binding to antibodies, bacterial toxins and viral receptors

   Författare :Stefan Gaunitz; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Carbohydrates are ubiquitous on the surface of all cells in mammals where they are involved in interactions with the surroundings (extracellular matrix), other cells (including self and non-self) and microbes (bacteria and virus). Carbohydrate-protein interactions in nature are often mediated via multivalent binding where the combined strength of multiple receptor- ligand interactions results in a binding that is highly specific and strong. LÄS MER

4. 18. Novel chemical and enzymatic routes to the generation of heparin- related polysaccarides

   Författare :Jianhui Rong; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; heparin; heparan sulphate; biosynthesis; 2-O-sulphotransferase; neo-glycosaminoglycan; conjugation; Biokemi; Biochemistry; Biokemi; Medical Biochemistry; medicinsk biokemi;

   Sammanfattning : Heparin and heparan sulphate (HS) are structurally related polysaccharides influencing various biological processes. The biological functions of heparin/HS largely depend on interactions between the negatively charged polysaccharide chains and a variety of proteins. LÄS MER

5. 19. Influence of Lactic Acid Fermentation on Enteropathogenic Bacteria. Growth Inhibition in Cereal Gruels and in Human Intestine

   Författare :Rose Kingamkono; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; intestinal mucosal permeability; enteropathogenic bacteria; enterotoxins; lactic acid fermentation;

   Sammanfattning : Togwa, a lactic-fermented cereal gruel, is widely consumed in Tanzania as a soft drink or used as a complementary food for young children. Studies were conducted to evaluate the potential of togwa to inhibit growth of common enteropathogenic bacteria and heat-labile (LT) enterotoxin production. LÄS MER

7. 20. Microfluidics based techniques for electrophysiological studies of cells

   Författare :Aikeremu Ahemaiti; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : This thesis work investigates the application of microfluidics to perform electrophysiological studies on cells, including investigations of the effect of cholesterol on the dynamic ion permeability of TRPV1 ion channels, and the application of a microfluidic device, the multifunctional pipette, in electrophysiological studies on brain slices. In the first part of this thesis, Chinese hamster ovary (CHO) cells overexpressing the TRPV1 ion channel were used in a dynamic ion permeability study, where the activation properties of the TRPV1 ion channel were investigated using the patch clamp technique after depletion of membrane cholesterol. LÄS MER