Sökning: "thermodynamics in enzyme catalysis"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden thermodynamics in enzyme catalysis.

  1. 1. On Catalytic Mechanisms for Rational Enzyme Design Strategies

    Författare :Charlotte Kürten; Per-Olof Syrén; Rudolf Allemann; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; catalytic mechanisms; terpene cyclase; triterpene cyclase; solvent dynamics; protein hydration; thermodynamics; quantum tunneling; polycyclization; natural compounds; ? ?-hydrolase; esterase; amidase; enzyme engineering; biocatalysis; Bioteknologi; Biotechnology;

    Sammanfattning : Enzymes enable life by promoting chemical reactions that govern the metabolism of all living organisms. As green catalysts, they have been extensively used in industry. However, to reach their full potential, engineering is often required, which can benefit from a detailed understanding of the underlying reaction mechanism. LÄS MER

  3. 2. Serine Hydrolase Selectivity : Kinetics and applications in organic and analytical chemistry

    Författare :Anders Hamberg; Karl Hult; Dick Janssen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; monoacylation of diols; kinetic resolution; thermodynamics in enzyme catalysis; enzyme engineering; Biochemistry; Biokemi;

    Sammanfattning : The substrate selectivities for different serine hydrolases were utilized in various applications, presented in papers I-VI. The articles are discussed in the thesis in view of the kinetics of the enzyme catalysis involved. LÄS MER

  4. 3. Redox Reactions of NO and O2 in Iron Enzymes : A Density Functional Theory Study

    Författare :Mattias Blomberg; Per Siegbahn; Adrian Mulholland; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzyme catalysis; redox reactions; cytochrome oxidase; nitric oxide reductase; hyponitrous acid anhydride; peroxynitrite; myoglobin; prostaglandin synthase; PGHS; NO; N2O; O2; CO; Chemical physics; Kemisk fysik;

    Sammanfattning : In the present thesis the density functional B3LYP has been used to study reactions of NO and O2 in redox active enzymes.Reduction of nitric oxide (NO) to nitrous oxide (N2O) is an important part in the bacterial energy conservation (denitrification). LÄS MER

  5. 4. Enzyme substrate solvent interactions : a case study on serine hydrolases

    Författare :Linda Fransson; Karl Hult; Jürgen Pleiss; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; esterase; specificity; Bioengineering; Bioteknik;

    Sammanfattning : Reaction rates and selectivities were measured for transacylation of fatty acid esters in solvents catalysed by Candida antarctica lipase B and by cutinase from Humicola insolens. With these enzymes classical water-based enzymology can be expanded to many different solvents allowing large variations in interaction energies between the enzymes, the substrates and the surrounding. LÄS MER

  7. 5. Sequence- and structure guided engineering of proteins and enzymes for biotechnology and health applications

    Författare :Karen Schriever; Per-Olof Syrén; Elton P. Hudson; Eric A. Gaucher; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein engineering; enzymology; ancestral sequence reconstruction; antigen engineering; structural biology; terpene cyclases; enzyme thermodynamics; biocatalysis; phosphatases; allosteric regulation; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are highly diverse and sophisticated biomolecules that represent a cornerstone of biological structure and function and have been exploited in man-made applications for thousands of years. Those proteins that facilitate chemical reactions at physiologically relevant time-scales are referred to as enzymes. LÄS MER