Sökning: "Biokemi"

Visar resultat 11 - 15 av 2077 avhandlingar innehållade ordet Biokemi.

  1. 11. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Författare :Lars O. Hansson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. LÄS MER

  3. 12. Chiral recognition on cellulases : The active sites of the enzymes are involved

    Författare :Hongbin Henriksson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Two cellobiohydrolases (CBH I and CBH II) and endoglucanase I (EG I) fromTrichoderma reesei, and CBH 58 from Phanerochaete chrysosporium were immobilized onsilica as chiral stationary phases (CSPs) in HPLC for enantioseparation of a group ofpharmaceutical compounds. CBH I and CBH 58 wore excellent chiral selectors for β-adrenergic blocking agents, whereas CBH II displayed good enantioselectivity for someother compounds The CBHs share the common feature that their active sites are located intunnels. LÄS MER

  4. 13. Structural studies on cytochrome bc1 complex from bovine heart mitochondria

    Författare :Momi Iwata; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Ubiquinol:cytochrome c oxidoreductase; membrane protein; X-ray crystallography; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The cytochrome bc1 complex (Ubiquinol:Cytochrome c oxidoreductase or Complex III) is an enzyme of the mitochondrial respiratory chain, crucial for aerobic metabolism. This enzyme catalyses the electron transfer from membrane-bound ubiquinol to soluble cytochrome c. LÄS MER

  5. 14. Catalysis by theta-class glutathione transferases : Substrate binding, product formation and product release

    Författare :Per Jemth; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Glutathione transferases (GSTs) are detoxication enzymes that catalyze theconjugation of glutathione to a large number of electrophilic molecules. In this waythey help to rid cells of potentially toxic substances. This thesis deals with thecatalysis afforded by Theta-class glutathione transferases. LÄS MER

  7. 15. Regulation, structure and function of glutathione reductase and its transcriptional regulator NtcA from the cyanobacterium Anabaena PCC 7120

    Författare :Fanyi Jiang; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Glutathione reductase (GR) is an important antioxidant enzyme in all aerobic life. Theenzyme was purified from cyanobacterium Anabaena PCC 7120 and overexpressed inEscherichia coli. It has physicochemical and kinetic properties similar to GR fromother sources, but unique features in the pyridine nucleotide-binding site. LÄS MER