Sökning: "sequence labelling"

Visar resultat 11 - 15 av 21 avhandlingar innehållade orden sequence labelling.

  1. 11. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Författare :Johan Nord; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER

  3. 12. Valorisation of Renewable Building Blocks via Transition Metal Catalysis – Glycerol- and Amino Acid Derived Compounds in Hydrogen Borrowing and RuAAC Reactions

    Författare :Anna Said Stålsmeden; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; amination; alkylation; RuAAC; 1 3-propanediol; renewable precursors; hydrogen borrowing; 1 2 3-triazole; solketal; glycerol; amino acid-derived azide;

    Sammanfattning : To a large extent, organic building blocks are today obtained from petroleum-based products. From an environmental point of view, biomass-derived compounds are more sustainable alternatives to such oil-derived molecules. The 12 principles of green chemistry describe how chemical processes can be improved in terms of sustainability. LÄS MER

  4. 13. Molecular studies of the insulin-like growth factor system in the brain : focus on growth, tumorigenesis and trauma

    Författare :Ann-Christin Sandberg Nordqvist; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :insulin-like growth factors; insulin-like growth factor binding proteins; brain; mRNA; meningioma; glioma; traumatic brain injury; in situ hybridization; Northern blothybridization;

    Sammanfattning : Insulin-like growth factor (IGF) 1 and -2 are synthesized by most tissues of the body and function as autocrine or paracrine agents in a tissue-specific manner. mRNA for IGF-1 and -2, as well as the IGF-binding proteins, are synthesized locally in various regions of the brain. LÄS MER

  5. 14. Cell cycle genes in chondrosarcoma. Involvement of the Id1 and p16 pathways

    Författare :Julia Asp; Göteborgs universitet; []
    Nyckelord :chondrosarcoma; articular chondrocytes; cell cycle; p16; Id1; helix-loop-helix;

    Sammanfattning : Chondrosarcoma is a malignant, cartilaginous tumour arising from bone. The wide variationin morphology, behaviour and clinical outcome raise the question whether chondrosarcomasare several different but closely related tumours rather than a single entity. LÄS MER

  7. 15. The role of Nkx3.2 and Gdf5 during zebrafish skeletal development

    Författare :Laura Waldmann; Tatjana Haitina; Abigail Tucker; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nkx3.2; Gdf5; zebrafish; jaw joint; joints; axial skeleton; appendicular skeleton; fin; enhancer conservation; CRISPR Cas9; OPT;

    Sammanfattning : The vertebrate skeleton is composed of bony and cartilaginous structures that are developed under the control of numerous genetic networks. The transcription factor Nkx3. LÄS MER