Sökning: "secretory pathway"

Visar resultat 6 - 10 av 81 avhandlingar innehållade orden secretory pathway.

  1. 6. Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae

    Författare :Jichen Bao; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; endoplasmic reticulum; oxidative stress; GLO3; protein secretion; transcriptomics; drug screening; Saccharomyces cerevisiae; SEC16;

    Sammanfattning : In the past few decades there has been an increasing demand of biopharmaceutical proteins in the market. Several types of cell factories are applied to produce different pharmaceutical proteins. LÄS MER

  3. 7. Structural studies of protein : protein complexes in the early secretory pathway

    Författare :Maria Svärd; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : In this thesis, components of three different protein-protein complexes in the early secretory pathway have been studied by NMR spectroscopy and X-ray crystallography, with the aim of giving detailed structural information on each protein and gaining a deeper insight into the molecular mechanisms by which each protein performs its function and interactions. The work has resulted in the three-dimensional structures of MCFD2, a protein involved in transport of coagulation factors V and VIII, Erv41p, a vesicle protein proposed to play a role in glycoprotein processing and transport, and the co-chaperone P58IPK. LÄS MER

  4. 8. Functional characterization of the secretory pathway and the role of COPI vesicles

    Författare :Johan Hiding; Göteborgs universitet; []
    Nyckelord :Golgi; Phosphoinositides; Fusion; COPI; ARF1; Proteomics; ER;

    Sammanfattning : In the secretory pathway, proteins synthesized in the endoplasmatic reticulum move through the Golgi apparatus in a cis-to-trans direction and then sorts into different types of vesicles at the trans-Golgi network. How intracellular transport takes place has been the focus of cell biology research for a long time. LÄS MER

  5. 9. Protein targeting, translocation and insertion in Escherichia coli : Proteomic analysis of substrate-pathway relationships

    Författare :Louise Baars; Jan-Willem de Gier; Jan-Maarten van Dijl; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein biogenesis; targeting; translocation; insertion; SecB; SecE; E. coli; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Approximately 10% of the open reading frames in the genome of the Gram-negative bacterium E. coli encodes secretory proteins, and 20% encodes integral inner membrane proteins (IMPs). LÄS MER

  7. 10. Structural studies of glycoprotein sorting and processing complexes in the early secretory pathway

    Författare :Dominik Possner; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The secretory pathway of a eukaryotic cell exerts a stringently regulated quality control system for the correct folding and transport of newly synthesised proteins and their subsequent transfer to their final destinations. While this pathway has been mostly studied in yeast, it has become increasingly clear that aberrations in its function are the main causes of a range of human disorders, and we are still in the process of gathering knowledge of the underlying molecular structures and mechanisms of the disease-causing agents in order to fully understand their impact. LÄS MER