Sökning: "protein array"

Visar resultat 16 - 20 av 229 avhandlingar innehållade orden protein array.

  1. 16. Method development for the analysis of protein interactions

    Författare :Despoina Kermpatsou; Ola Söderberg; Maréne Landström; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation assay PLA ; strand displacement; proximity-dependent initiation of hybridisation chain reaction proxHCR ; protein complexes; rolling circle amplification RCA ; MolBoolean; vitamin D; 1; 25D3-MARRS receptor; PDIA3; ERp57; CYP24A1; c-Myc; prostate cancer; Molekylär cellbiologi; Molecular Cellbiology; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Biological processes take place through interactions between macromolecules, such as nucleic acids and proteins. It is, therefore, fundamental to understand the functions of proteins and how they form complexes in order to carry out their role. LÄS MER

  3. 17. Epitope mapping of antibodies towards human protein targets

    Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

    Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

  4. 18. Functional characterization of interactions between the flavivirus NS5 protein and PDZ proteins of the mammalian host

    Författare :Karin Ellencrona; Magnus Johansson; Andrew Davidson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Tick-borne encephalitis virus TBEV ; West-Nile virus WNV ; Dengue virus DENV ; flavivirus; PDZ domains; ZO-2; ZO-1; RIMS2; Scribble; Interferon; JAK-STAT signaling; PDZ array; virus-host protein interaction; Genetics; Genetik; molekylärgenetik; Molecular Genetics;

    Sammanfattning : Flaviviruses are found all over the world and affect and infect millions of people every year. Flavivirus infection can lead to severe clinical outcomes resulting in neuronal damages e.g. Tick-borne encephalitis virus (TBEV), or severe hemorrhagic fevers e. LÄS MER

  5. 19. Mammalian protein expression and characterization tools for next generation biologics

    Författare :Niklas Thalén; Johan Rockberg; Kerstin Otte; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CHO; Cell line engineering; Protein engineering; GFP; Cell display; Biotechnology; Bioteknologi;

    Sammanfattning : Protein therapeutics are increasingly important for modern medicine. Novel recombinant proteins developed today can bind towards their target with high specificity and with low adverse effect. This has enabled the treatment of diseases that for a few years ago were deemed uncurable. LÄS MER

  7. 20. Microfluidic Methods for Protein Microarrays

    Författare :Michael Hartmann; Johan Roeraade; Mike Taussig; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Protein microarray technology has an enormous potential for in vitro diagnostics (IVD)1. Miniaturized and parallelized immunoassays are powerful tools to measure dozens of parameters from minute amounts of sample, whilst only requiring small amounts of reagent. LÄS MER