Sökning: "Larry Gold"

Hittade 4 avhandlingar innehållade orden Larry Gold.

1. 1. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

   Författare :Simon Fredriksson; Larry Gold; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

   Sammanfattning : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. LÄS MER

3. 2. Epitope mapping of antibodies towards human protein targets

   Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

   Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

4. 3. Development and Application of Triple Specific Proximity Ligation Assays (3PLA)

   Författare :Edith Schallmeiner; Ulf Landegren; Larry Gold; Uppsala universitet; []
   Nyckelord :Molecular medicine; proxmity ligation; method development; cell siganling; Molekylärmedicin;

   Sammanfattning : After the completion of the human genome project the human genome was annotated with the surprisingly small amount of 24 000 (www.ensemble.com) genes. LÄS MER

5. 4. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications

   Författare :Mikael Åstrand; Sophia Hober; John Löfblom; Larry Gold; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Protein G; ERBB2; ERBB3; Directed evolution; phage display; staphylococcal display; bispecific; Bioteknologi; Biotechnology;

   Sammanfattning : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. LÄS MER