Sökning: "prokaryotic"

Visar resultat 6 - 10 av 86 avhandlingar innehållade ordet prokaryotic.

1. 6. Expression of recombinant neutralizing anti-HIV-1 antibodies in bacteria and eukaryotic cells

   Författare :Fayezeh Yari; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Phage display; vector; monoclonal antibody; antigen; eukaryotic; prokaryotic; transfection; selection; transient expression; stable cell line; neutralizing antibody; expression; HIVA-1; gp41; gp120;

   Sammanfattning : The main purpose of this study was to investigate and optimize techniques for eukaryotic expression of antibody molecules (IgG, Fab and scFV-ê formats), derived from an immune phage displayed Fab library and reactive against HIV-1 glycoproteins gp120 and gp41. Light and heavy-chain (Fd) DNA from a number of Fab clones were transferred from a prokaryotic phagemid vectors to mammalian expression vectors. LÄS MER

3. 7. Structural and functional studies of nutrient and drug uptake systems

   Författare :Fatma Guettou; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Orally ingested nutrients and drugs are selectively absorbed from our small intestines into the bloodstream through various membrane-integrated transporters. The present study focuses mainly on a specific absorption route, namely the proton dependent oligopeptide transporters (POTs). LÄS MER

4. 8. Evolution of biological systems linked to complex cell architectures in Planctomycetota

   Författare :Emil Hägglund; Siv Andersson; Damien Devos; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Planctomcyetota; Cellular complexity; Gemmataceae; Phagocytocis; Serine Threonine kinase; Anammox; Hydrazine Synthase; Hydroxylamine oxidoreductase; Heme biosynthesis; Phylogenetics; Biologi med inriktning mot molekylär evolution; Biology with specialization in Molecular Evolution;

   Sammanfattning : The eukaryotic cells have a nucleus and membrane-enclosed organelles for functions such as energy production, whereas the prokaryotic cells have a cell wall but no intracellular membrane-bound structures. Species of the Planctomycetota phylum are classified as bacteria but have diverse and complex cell architectures. LÄS MER

5. 9. Ecology of fungal denitrifiers in terrestrial ecosystems : global patterns and effects of management in agricultural soils

   Författare :Yvonne Bösch; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

   Sammanfattning : Denitrification is the dominant source of nitrous oxide (N2O) from terrestrial ecosystems, a potent greenhouse gas and stratospheric ozone-depleting agent. Fungi can perform denitrification and terminate the process with N2O, making them a potentially important source of N2O. LÄS MER

7. 10. Structure, prediction, evolution and genome wide studies of membrane proteins

   Författare :Erik Granseth; Arne Elofsson; Gunnar von Heijne; Mark Sansom; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Theoretical chemistry; Teoretisk kemi; biokemi; Biochemistry;

   Sammanfattning : α-helical membrane proteins constitute 20-30% of all proteins in a cell and are involved in many essential cellular functions. The structure is only known for a few hundred of them, which makes structural models important. LÄS MER