Sökning: "HIVA-1"

Hittade 1 avhandling innehållade ordet HIVA-1.

1. 1. Expression of recombinant neutralizing anti-HIV-1 antibodies in bacteria and eukaryotic cells

   Författare :Fayezeh Yari; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Phage display; vector; monoclonal antibody; antigen; eukaryotic; prokaryotic; transfection; selection; transient expression; stable cell line; neutralizing antibody; expression; HIVA-1; gp41; gp120;

   Sammanfattning : The main purpose of this study was to investigate and optimize techniques for eukaryotic expression of antibody molecules (IgG, Fab and scFV-ê formats), derived from an immune phage displayed Fab library and reactive against HIV-1 glycoproteins gp120 and gp41. Light and heavy-chain (Fd) DNA from a number of Fab clones were transferred from a prokaryotic phagemid vectors to mammalian expression vectors. LÄS MER