Sökning: "molecule fluorescence"

Visar resultat 1 - 5 av 146 avhandlingar innehållade orden molecule fluorescence.

  1. 1. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Författare :Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Sammanfattning : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. LÄS MER

  3. 2. First As Probe, Then As Function - Fluorescence in Bio-inspired Nanotechnology

    Författare :Jonas Hannestad; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; self-assembly; lipid membrane; bio-inspired; FRET; light-harvesting; spectroscopy.; fluorescence; nanotechnology; single molecule fluorescence spectroscopy; DNA;

    Sammanfattning : In this thesis, I demonstrate how fluorescence can be used in the context of bio-inspired nanotechnology, both as an indirect probe and as a function in itself. By combining principles and molecules from three different bio-molecular systems, DNA, bacterial light-harvesting complexes and cell membranes, I have constructed nano- and microscale systems for long-range excitation energy transfer, light-harvesting and reaction control. LÄS MER

  4. 3. Design, Synthesis and Characterization of Small Molecule Inhibitors and Small Molecule : Peptide Conjugates as Protein Actors

    Författare :Jonas Nilsson; Lars Baltzer; Ingemar Kvarnström; Thomas Olsson; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Synthetic receptor; Peptide inhibitor conjugate; Peptide protein surface interactions; General Lead Discovery Library; Combinatorial Chemistry; Solid Phase Chemistry; Targeted Library; Thrombin inhibitor; Fluorescence; Surface Plasmon Resonance; Organic chemistry; Organisk kemi;

    Sammanfattning : This thesis describes different aspects of protein interactions. Initially the function of peptides and their conjugates with small molecule inhibitors on the surface of Human Carbonic Anhydrase isoenzyme II (HCAII) is evaluated. LÄS MER

  5. 4. Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications

    Författare :Gustav Persson; Jerker Widengren; Jörg Enderlein; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence; spectroscopy; microscopy; modulated excitation; intensity modulation; fluorescence correlation spectroscopy; transient states; molecular kinetics; Physics; Fysik;

    Sammanfattning : This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. LÄS MER

  7. 5. Polarization portraits of lightharvesting antennas: from single molecule spectroscopy to imaging

    Författare :Rafael Camacho Dejay; Kemisk fysik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Energy transfer; Single molecule spectroscopy; Exciton; Fluorescence microscopy; Fluorescence polarization; Light-harvesting; Chromophore; Conjugated Polymer; Photosynthesis;

    Sammanfattning : Multichromophoric systems are very important in photosynthesis and any device that uses solar energy for its operation. This is because multichromophoric light-harvesting antennas are responsible for the absorption of light and the efficient transfer of the absorbed energy toward distinct places where it is to be used or stored. LÄS MER