Sökning: "modulated excitation"
Visar resultat 1 - 5 av 18 avhandlingar innehållade orden modulated excitation.
1. Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications
Sammanfattning : This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. LÄS MER
2. Transient State Fluorescence Microscopy - method development and biological applications : Exploiting the dark states of fluorophores to measure oxygen concentrations, redox state, Förster resonance energy transfer and membrane viscosity
Sammanfattning : Due to their long lifetime, triplet, redox and other transient states of fluorophores are highly sensitive to the micro-environment. Imaging their spatial distribution in biological samples can therefore help answer interesting questions about the metabolism, molecular interactions and dynamics in living cells. LÄS MER
3. Monitoring Proton Exchange and Triplet States with Fluorescence
Sammanfattning : Fluorescent molecules commonly shift to transient dark states, induced bylight or triggered by chemical reactions. The transient dark states can beused as probes of the local environment surrounding the fluorescent molecules,and are therefore attractive for use in biomolecular applications. LÄS MER
4. Concepts and biomedical applications of excitation-modulated transient state monitoring of fluorescence emitters
Sammanfattning : Fluorescence methods have developed very strongly in the last decade, allowing single- molecule detection sensitivity, high specificity, high time- and spatial resolution, as well as high readout speeds. Together, this makes fluorescencea very central readout modality for biomolecular and cellular studies. LÄS MER
5. Time-Varying Excitation in Fluorescence Spectroscopy for Biological Applications
Sammanfattning : The focus of this thesis is to explore and use the benefits of time-varying excitation in fluorescence spectroscopy for studies of biomolecular dynamics. Two new techniques taking advantage of modulated excitation are presented. LÄS MER