Sökning: "molecular immobilization"

Visar resultat 21 - 25 av 53 avhandlingar innehållade orden molecular immobilization.

  1. 21. Nanoporous Aluminum Oxide – A Promising Support for Modular Enzyme Reactors

    Författare :Marcus Kjellander; Gunnar Johansson; Gunnar Henriksson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; nanoporous aluminum oxide; immobilized enzymes; bioreactor; Biokemi; Biochemistry;

    Sammanfattning : Nanoporous alumina is a rather newly characterized material that so far has found limited use in the construction of bioreactors. The material has many advantages compared to conventional immobilization matrices. I have investigated its use in flow-through bioreactors. LÄS MER

  3. 22. Chromatographic characterization of the human red cell glucose transporter Glut1 in the cells, in membrane vesicles and in proteoliposomes

    Författare :Andreas Lundqvist; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The human glucose transporter Glut1 is used by many cells, especially red blood cells andbrain cell, to take up glucose and other necessary metabolites. Gentle chromatographic methods can be applied for analyses of Glut1 activities in lipid environments similar to the native one. LÄS MER

  4. 23. Fluorescence-based ligand assays for protein detection using affibody affinity proteins

    Författare :Björn Renberg; Amelie Eriksson Karlström; Per-Åke Nygren; Jörg Hoheisel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; biosensors; FRET; immobilisation; solid-phase synthesis; protein microarrays; Biochemistry; Biokemi;

    Sammanfattning : The detection and quantification of biomolecules, and proteins in particular, are of great interest since these molecules are of fundamental importance to our well-being. Body fluids, as for instance human blood, are well suited for sampling of protein levels. LÄS MER

  5. 24. Progress of Weak Affinity Chromatography as a Tool in Drug Development

    Författare :Elinor Meiby; Sten Ohlson; Manfred Raida; Linnéuniversitetet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; weak affinity chromatography; fragment based drug discovery; fragment screening; thrombin; trypsin; cyclin G-associated kinase; Hsp90; Pin1; cyclooxygenase; lipodisks; Biokemi; Biochemistry;

    Sammanfattning : Weak Affinity Chromatography (WAC) is a technology that was developed to analyse weak (KD > 10-5 M) although selective interactions between biomolecules. The focus of this thesis was to develop this method for various applications in the drug development process.Fragment Based Drug Discovery is a new approach in finding new small molecular drugs. LÄS MER

  7. 25. Chromatographic studies of interactions between solutes, lipid bilayers and the glucose transporter Glut1

    Författare :Yanxiao Zhang; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : It is advantageous to analyze solute interactions with biological membranes by chromatography on stationary phases that mimic the membrane structures. In the present work, liposomes, proteoliposomes, human red cells and red cell membrane vesicles were immobilized for analyses ofinteractions between solutes (peptides, drugs, inhibitors and glucose), lipid bilayers and theglucose transporter Glut1. LÄS MER