Sökning: "mRNA quantification"

Visar resultat 11 - 15 av 43 avhandlingar innehållade orden mRNA quantification.

  1. 11. Detection and Sequencing of Amplified Single Molecules

    Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

  3. 12. Identification and evaluation of growth hormone antagonists

    Författare :Linda Rosengren; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : States in which Growth hormone (GH) levels are elevated have been implicated in several physiological disorders such as acromegaly, diabetic complications and progression of malignancies. Classical treatments for acromegaly are surgery and radiotherapy but also pharmacological therapies exist. LÄS MER

  4. 13. Cancer proteogenomics : connecting genotype to molecular phenotype

    Författare :Ioannis Siavelis; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The central dogma of molecular biology describes the one-way road from DNA to RNA and finally to protein. Yet, how this flow of information encoded in DNA as genes (genotype) is regulated in order to produce the observable traits of an individual (phenotype) remains unanswered. Recent advances in high-throughput data, i.e. LÄS MER

  5. 14. Evaluation, synthesis and imaging of therapeutic oligonucleotides

    Författare :Rouven Stulz; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : A new method for the ³⁴S isotope labelling of phosphorothioate oligonucleotides is reported which allows a practical synthesis of site specifically labeled oligonucleotides. Several applications of this method are reported. LÄS MER

  7. 15. Standardization and application of quantitative PCR methods in patients with hematological malignancies

    Författare :Maria Malec; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Polymerase chain reaction; RQ-PCR; Minimal Residual Disease; Acute leukemia; Hodgkin s Lymphoma; cytokines;

    Sammanfattning : Using modem combination chemotherapy regimens, it has been possible to increase the rate of complete remission (CR) in acute lymphoblastic (ALL) and acute myeloid leukemia (AML). The major reason for treatment failure in acute leukemia (AL) is the resistance of leukemic blasts to chemotherapeutic agents, which is accompanied by an inability of the immune system to eradicate residual leukemic cells surviving initial treatment. LÄS MER