Sökning: "mRNA quantification"

Visar resultat 16 - 20 av 43 avhandlingar innehållade orden mRNA quantification.

  1. 16. Mapping the proteome with data-driven methods: A cycle of measurement, modeling, hypothesis generation, and engineering

    Författare :Filip Buric; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; tensor factorization; mass spectrometry; model interpretation; sequence feature engineering; deep learning; proteomics; machine learning; data-independent acquisition; feature learning;

    Sammanfattning : The living cell exhibits emergence of complex behavior and its modeling requires a systemic, integrative approach if we are to thoroughly understand and harness it. The work in this thesis has had the more narrow aim of quantitatively characterizing and mapping the proteome using data-driven methods, as proteins perform most functional and structural roles within the cell. LÄS MER

  3. 17. Deregulation and editing of microRNAs in metastatic prostate cancer

    Författare :Gjendine Voss; Avdelningen för translationell cancerforskning; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; prostate cancer; bone metastasis; microRNA; RNA editing; A-to-I editing; ADAR; miR-96; miR-379;

    Sammanfattning : Prostate cancer bone metastasis is still not fully understood, and increased knowledge could aid in the development of better treatment options for patients in the future. This thesis focuses on microRNAs (miRNAs), which modulate gene expression in healthy and tumour cells. LÄS MER

  4. 18. Genes involved in Parkinson's disease : focus on mitochondrial and detoxifying enzymes

    Författare :Anna Anvret; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Parkinson's disease is a common progressive neurodegenerative disorder which mostly affects the elderly population, with a prevalence of more than 1.5% in the population over the age of 65 years. Clinical motor symptoms are mainly caused by degeneration of dopamine neurons in substantia nigra pars compacta. LÄS MER

  5. 19. Methodological studies of eicosanoid enzyme gene expression in vascular endothelium exposed to biomechanical stress

    Författare :Roya Doroudi; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; prostanoids; endothelium; quantitative RT-PCR; perfusion model; shear stress; intraluminal pressure; immediate-early genes;

    Sammanfattning : Prostaglandins are the major eicosanoids synthesized by vascular endothelial cells, and the vasculature is an important target organ for their bioactive metabolites. In vitro studies have shown that biomechanical forces are important regulators of vascular expression of prostanoid enzymes and release of metabolites. LÄS MER

  7. 20. Gene expression profiling with real-time PCR

    Författare :Anders Ståhlberg; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; single cell biology; reverse transcription; LightUp probe; cancer diagnostics; real-time PCR; gene expression profiling; tumor biology;

    Sammanfattning : The sequence of the entire human genome is available today, and this information can be used to understand, characterize, and treat complex diseases such as cancer. Many cellular functions related to survival, growth and differentiation are reflected in the gene expression pattern, and the ability to quantify transcription levels of specific genes is, and always has been, central to research on gene function. LÄS MER